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Curr Genet

Title:		Special type of pheromone-induced invasive growth in Saccharomyces cerevisiae
Author(s):		Frydlova I; Basler M; Vasicova P; Malcova I; Hasek J;
Address:		"Institute of Microbiology of AS CR, v.v.i, Videnska 1083, 142 20 Prague 4, Czech Republic"
Journal Title:		Curr Genet
Year:		2007
Volume:		20070717
Issue:		2
Page Number:		87 - 95
DOI:		10.1007/s00294-007-0141-2
ISSN/ISBN:		0172-8083 (Print) 0172-8083 (Linking)
Abstract:		"The ability to invade a solid substrate is an important phenomenon due to its connection with pathogenic activity of fungi. We report here on invasion displayed by MATalpha cells of Saccharomyces cerevisiae lacking Isw2p, a subunit of the ISW2 chromatin remodelling complex. We found that on minimal medium, where the isw2Delta MATalpha mutant is not invasive, additional absence of another ISW2 complex subunit, Dls1p or Dpb4p, promoted invasion. Our microarray data showed that derepression of MAT a-specific genes caused by absence of Isw2p is very low. Their expression is increased only by the autocrine activation of the mating pathway. Invasion of isw2Delta MATalpha cells thus resembles the pheromone-induced invasion, including dependence on Fig2p. We show here that another pheromone-induced protein, mating agglutinin Aga1p, can play a role in the agar adhesion necessary for invasion. In contrast with MAT a-cells invading agar under low alpha-pheromone concentration, the invasive growth of isw2Delta cells specifically requires Fus3 kinase. Its function in the invasion of isw2Delta MATalpha cells cannot be completely substituted by Kss1 kinase, which plays a basic role in invasive growth signalling. We suggest that partial dependence of the isw2Delta MATalpha invasion on Fus3p and Aga1p corresponds to a weaker pheromone response of this mutant"
Keywords:		"Adenosine Triphosphatases/genetics/metabolism Cell Adhesion Molecules/genetics/metabolism Gene Deletion Genes, Fungal MAP Kinase Signaling System Mitogen-Activated Protein Kinases/genetics/metabolism Models, Biological Nuclear Proteins/genetics/metabolism;"
Notes:		"MedlineFrydlova, Ivana Basler, Marek Vasicova, Pavla Malcova, Ivana Hasek, Jiri eng Research Support, Non-U.S. Gov't 2007/07/20 Curr Genet. 2007 Aug; 52(2):87-95. doi: 10.1007/s00294-007-0141-2. Epub 2007 Jul 17"