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Insect Biochem Mol Biol

Title:		"Reverse chemical ecology-based approach leading to the accidental discovery of repellents for Rhodnius prolixus, a vector of Chagas diseases refractory to DEET"
Author(s):		Franco TA; Xu P; Brito NF; Oliveira DS; Wen X; Moreira MF; Unelius CR; Leal WS; Melo ACA;
Address:		"Universidade Federal do Rio de Janeiro, Instituto de Quimica, 21941-909, Rio de Janeiro, RJ, Brazil; Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA. Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA. Universidade Federal do Rio de Janeiro, Instituto de Quimica, 21941-909, Rio de Janeiro, RJ, Brazil. Universidade Federal do Rio de Janeiro, Instituto de Quimica, 21941-909, Rio de Janeiro, RJ, Brazil; Instituto Nacional de Ciencia e Tecnologia em Entomologia Molecular, Rio de Janeiro, RJ, Brazil. Faculty of Health and Life Sciences, Linnaeus University, Kalmar, Sweden. Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA. Electronic address: wsleal@ucdavis.edu. Universidade Federal do Rio de Janeiro, Instituto de Quimica, 21941-909, Rio de Janeiro, RJ, Brazil; Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA; Instituto Nacional de Ciencia e Tecnologia em Entomologia Molecular, Rio de Janeiro, RJ, Brazil. Electronic address: anamelo@iq.ufrj.br"
Journal Title:		Insect Biochem Mol Biol
Year:		2018
Volume:		20181026
Issue:
Page Number:		46 - 52
DOI:		10.1016/j.ibmb.2018.10.004
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Print) 0965-1748 (Linking)
Abstract:		"Rhodnius prolixus is one of the most important vectors of Chagas disease in Central and South America for which repellents and attractants are sorely needed. Repellents like DEET, picaridin, and IR3535 are widely used as the first line of defense against mosquitoes and other vectors, but they are ineffective against R. prolixus. Our initial goal was to identify in R. prolixus genome odorant receptors sensitive to putative sex pheromones. We compared gene expression of 21 ORs in the R. prolixus genome, identified 4 ORs enriched in male (compared with female) antennae. Attempts to de-orphanize these ORs using the Xenopus oocyte recording system showed that none of them responded to putative sex pheromone constituents. One of the them, RproOR80, was sensitive to 4 compounds in our panel of 109 odorants, namely, 2-heptanone, gamma-octalactone, acetophenone, and 4-methylcychohexanol. Interestingly, these compounds, particularly 4-methylcyclohexanol, showed strong repellency activity as indicated not only by a significant decrease in residence time close to a host, but also by a remarkable reduction in blood intake. 4-Methylcyclohexanol-elicited repellency activity was abolished in RNAi-treated insects. In summary, our search for pheromone receptors led to the discovery of repellents for R. prolixus"
Keywords:		Acetophenones/pharmacology Animals Arthropod Antennae/*drug effects Chagas Disease/prevention & control Cyclohexanols/*pharmacology Female Insect Repellents/pharmacology Insect Vectors/drug effects Ketones/pharmacology Lactones/pharmacology Male Receptors;
Notes:		"MedlineFranco, Thiago A Xu, Pingxi Brito, Nathalia F Oliveira, Daniele S Wen, Xiaolan Moreira, Monica F Unelius, C Rikard Leal, Walter S Melo, Ana C A eng R01 AI095514/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2018/11/08 Insect Biochem Mol Biol. 2018 Dec; 103:46-52. doi: 10.1016/j.ibmb.2018.10.004. Epub 2018 Oct 26"