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J Exp Biol

Title:		Perfusion with cAMP analogue affects pheromone-sensitive trichoid sensilla of the hawkmoth Manduca sexta in a time-dependent manner
Author(s):		Flecke C; Nolte A; Stengl M;
Address:		"Biologie, Tierphysiologie, Philipps-Universitat Marburg, 35032 Marburg, Germany"
Journal Title:		J Exp Biol
Year:		2010
Volume:		213
Issue:		5
Page Number:		842 - 852
DOI:		10.1242/jeb.032839
ISSN/ISBN:		1477-9145 (Electronic) 0022-0949 (Linking)
Abstract:		"Octopamine causes time-dependent disadaptation of pheromone-sensitive olfactory receptor neurons (ORNs) of Manduca sexta. Because the majority of insect octopamine receptors are positively coupled to adenylyl cyclases we examined whether cyclic adenosine monophosphate (cAMP) mimics octopamine-dependent modulation of pheromone transduction in a time-dependent manner. Long-term tip recordings of single trichoid sensilla of Manduca sexta were performed during three zeitgeber times (ZTs, ZT 0=lights on), while stimulating the sensilla with two doses of the main pheromone component bombykal in a non-adapting protocol. The membrane-permeable cAMP analogue 8bcAMP increased the normalized sensillar potential amplitude in a time- and bombykal dose-dependent way. At the higher bombykal dose only, the applied 8bcAMP antagonized an endogenous decrease in the mean sensillar potential amplitude at ZT 1-4 and ZT 8-11 when ORNs were adapted but not at ZT 22-1, when ORNs were sensitized. In contrast to octopamine, 8bcAMP did not consistently affect the initial pheromone-dependent action potential frequency, the phasic/tonic response pattern, or the time-dependent shift to lower mean action potential frequencies at ZT 8-11. Furthermore, 8bcAMP increased the spontaneous action potential frequency time dependently, but differently from octopamine. In conclusion, our results show that cAMP only partly mimics the octopamine-dependent disadaptation of olfactory receptor neurons during photophase, apparently due to another missing octopamine-dependent synergistic factor such as defined intracellular calcium levels"
Keywords:		8-Bromo Cyclic Adenosine Monophosphate/*pharmacology Action Potentials/drug effects/radiation effects Alkadienes/pharmacology Animals In Vitro Techniques Light Male Manduca/*drug effects/*physiology/radiation effects Octopamine/pharmacology Olfactory Rece;
Notes:		"MedlineFlecke, Christian Nolte, Andreas Stengl, Monika eng Research Support, Non-U.S. Gov't England 2010/02/16 J Exp Biol. 2010 Mar 1; 213(5):842-52. doi: 10.1242/jeb.032839"