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Mol Cell Biol

Title:		"Regulation by the yeast mating-type locus of STE12, a gene required for cell-type-specific expression"
Author(s):		Fields S; Herskowitz I;
Address:		"Department of Microbiology, State University of New York at Stony Brook 11794"
Journal Title:		Mol Cell Biol
Year:		1987
Volume:		7
Issue:		10
Page Number:		3818 - 3821
DOI:		10.1128/mcb.7.10.3818-3821.1987
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"The STE12 gene of the yeast Saccharomyces cerevisiae is necessary for RNA synthesis from two sets of cell-type-specific genes. We isolated a recombinant plasmid that carries the STE12 gene by complementation of the mating defect of ste12 cells. The DNA of the cloned gene was used to disrupt the chromosomal STE12 gene and to identify the STE12 transcript. We show that the STE12 transcript level is repressed 5- to 10-fold in a/alpha cells. The STE12 product thus acts to promote diploidy by activating expression of the two sets of genes necessary for mating, and then its synthesis is repressed by products unique to the diploid cell type"
Keywords:		"Cell Differentiation Cloning, Molecular DNA Restriction Enzymes Gene Expression Regulation *Genes, Fungal *Genes, Mating Type, Fungal Mating Factor Peptides/*genetics RNA, Fungal/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/*genetics;"
Notes:		"MedlineFields, S Herskowitz, I eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1987/10/01 Mol Cell Biol. 1987 Oct; 7(10):3818-21. doi: 10.1128/mcb.7.10.3818-3821.1987"