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Microorganisms

Title:		Rosenbergiella meliponini D21B Isolated from Pollen Pots of the Australian Stingless Bee Tetragonula carbonaria
Author(s):		Farlow AJ; Rupasinghe DB; Naji KM; Capon RJ; Spiteller D;
Address:		"Chemical Ecology/Biological Chemistry, Department of Biology, University of Konstanz, Universitatsstrasse 10, 78457 Konstanz, Germany. Centre for Drug Discovery, Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, Brisbane, QLD 4072, Australia"
Journal Title:		Microorganisms
Year:		2023
Volume:		20230412
Issue:		4
Page Number:		-
DOI:		10.3390/microorganisms11041005
ISSN/ISBN:		2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking)
Abstract:		"Rosenbergiella bacteria have been previously isolated predominantly from floral nectar and identified in metagenomic screenings as associated with bees. Here, we isolated three Rosenbergiella strains from the robust Australian stingless bee Tetragonula carbonaria sharing over 99.4% sequence similarity with Rosenbergiella strains isolated from floral nectar. The three Rosenbergiella strains (D21B, D08K, D15G) from T. carbonaria exhibited near-identical 16S rDNA. The genome of strain D21B was sequenced; its draft genome contains 3,294,717 bp, with a GC content of 47.38%. Genome annotation revealed 3236 protein-coding genes. The genome of D21B differs sufficiently from the closest related strain, Rosenbergiella epipactidis 2.1A, to constitute a new species. In contrast to R. epipactidis 2.1A, strain D21B produces the volatile 2-phenylethanol. The D21B genome contains a polyketide/non-ribosomal peptide gene cluster not present in any other Rosenbergiella draft genomes. Moreover, the Rosenbergiella strains isolated from T. carbonaria grew in a minimal medium without thiamine, but R. epipactidis 2.1A was thiamine-dependent. Strain D21B was named R. meliponini D21B, reflecting its origin from stingless bees. Rosenbergiella strains may contribute to the fitness of T. carbonaria"
Keywords:		2-phenylethanol genome mass spectrometry microbial symbionts phylogeny secondary metabolite vitamins volatile compounds;
Notes:		"PubMed-not-MEDLINEFarlow, Anthony J Rupasinghe, Darshani B Naji, Khalid M Capon, Robert J Spiteller, Dieter eng Switzerland 2023/04/28 Microorganisms. 2023 Apr 12; 11(4):1005. doi: 10.3390/microorganisms11041005"