| Title: | Sensory Metabolite Profiling in a Date Pit Based Coffee Substitute and in Response to Roasting as Analyzed via Mass Spectrometry Based Metabolomics |
| Author(s): | Farag MA; Otify AM; El-Sayed AM; Michel CG; ElShebiney SA; Ehrlich A; Wessjohann LA; |
| Address: | "Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt. mohamed.farag@pharma.cu.edu.eg. Department of Chemistry, School of Sciences and Engineering, The American University in Cairo, New Cairo 11835, Egypt. mohamed.farag@pharma.cu.edu.eg. Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt. Toxicology and Narcotics Department, National Research Centre, Giza 12622, Egypt. Leibniz Institute of Plant Biochemistry, Dept. Bioorganic Chemistry, Weinberg 3, D-06120 Halle (Saale), Germany. Leibniz Institute of Plant Biochemistry, Dept. Bioorganic Chemistry, Weinberg 3, D-06120 Halle (Saale), Germany. wessjohann@ipb-halle.de" |
| DOI: | 10.3390/molecules24183377 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Interest in developing coffee substitutes is on the rise, to minimizing its health side effects. In the Middle East, date palm (Phoenix dactylifera L.) pits are often used as a coffee substitute post roasting. In this study, commercially-roasted date pit products, along with unroasted and home-prepared roasted date pits, were subjected to analyses for their metabolite composition, and neuropharmacological evaluation in mice. Headspace SPME-GCMS and GCMS post silylation were employed for characterizing its volatile and non-volatile metabolite profile. For comparison to roasted coffee, coffee product was also included. There is evidence that some commercial date pit products appear to contain undeclared additives. SPME headspace analysis revealed the abundance of furans, pyrans, terpenoids and sulfur compounds in roasted date pits, whereas pyrroles and caffeine were absent. GCMS-post silylation employed for primary metabolite profiling revealed fatty acids' enrichment in roasted pits versus sugars' abundance in coffee. Biological investigations affirmed that date pit showed safer margin than coffee from its LD(50), albeit it exhibits no CNS stimulant properties. This study provides the first insight into the roasting impact on the date pit through its metabolome and its neuropharmacological aspects to rationalize its use as a coffee substitute" |
| Keywords: | Animals Beverages/*analysis Coffee/*chemistry Male *Mass Spectrometry/methods *Metabolome *Metabolomics/methods Mice Phytochemicals/*analysis Volatile Organic Compounds Phoenix dactylifera coffee date palm multivariate analysis volatiles; |
| Notes: | "MedlineFarag, Mohamed A Otify, Asmaa M El-Sayed, Aly M Michel, Camilia G ElShebiney, Shaimaa A Ehrlich, Anja Wessjohann, Ludger A eng Switzerland 2019/09/20 Molecules. 2019 Sep 17; 24(18):3377. doi: 10.3390/molecules24183377" |
