| Title: | Pheromone-induced morphogenesis and gradient tracking are dependent on the MAPK Fus3 binding to Galpha |
| Author(s): | Errede B; Vered L; Ford E; Pena MI; Elston TC; |
| Address: | "Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 errede@email.unc.edu. Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599. Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599. Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599" |
| ISSN/ISBN: | 1939-4586 (Electronic) 1059-1524 (Print) 1059-1524 (Linking) |
| Abstract: | "Mitogen-activated protein kinase (MAPK) pathways control many cellular processes, including differentiation and proliferation. These pathways commonly activate MAPK isoforms that have redundant or overlapping function. However, recent studies have revealed circumstances in which MAPK isoforms have specialized, nonoverlapping roles in differentiation. The mechanisms that underlie this specialization are not well understood. To address this question, we sought to establish regulatory mechanisms that are unique to the MAPK Fus3 in pheromone-induced mating and chemotropic fate transitions of the budding yeast Saccharomyces cerevisiae. Our investigations reveal a previously unappreciated role for inactive Fus3 as a potent negative regulator of pheromone-induced chemotropism. We show that this inhibitory role is dependent on inactive Fus3 binding to the alpha-subunit of the heterotrimeric G-protein. Further analysis revealed that the binding of catalytically active Fus3 to the G-protein is required for gradient tracking and serves to suppress cell-to-cell variability between mating and chemotropic fates in a population of pheromone-responding cells" |
| Keywords: | GTP-Binding Protein alpha Subunits/*metabolism MAP Kinase Signaling System/drug effects Mitogen-Activated Protein Kinases/*metabolism Morphogenesis/drug effects Pheromones/*pharmacology Saccharomyces cerevisiae/drug effects/enzymology/metabolism Saccharom; |
| Notes: | "MedlineErrede, Beverly Vered, Lior Ford, Eintou Pena, Matthew I Elston, Timothy C eng GM079271/GM/NIGMS NIH HHS/ R01 GM079271/GM/NIGMS NIH HHS/ T32 GM008570/GM/NIGMS NIH HHS/ R01 GM114136/GM/NIGMS NIH HHS/ GM114136/GM/NIGMS NIH HHS/ GM084071/GM/NIGMS NIH HHS/ R01 GM084071/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural 2015/07/17 Mol Biol Cell. 2015 Sep 15; 26(18):3343-58. doi: 10.1091/mbc.E15-03-0176. Epub 2015 Jul 15" |
