| Title: | "The effect of inactivation of aldehyde dehydrogenase on pheromone production by a gut bacterium of an invasive bark beetle, Dendroctonus valens" |
| Author(s): | Cao Q; Koski TM; Li H; Zhang C; Sun J; |
| Address: | "College of Forestry, Hebei Agricultural University, Baoding, Hebei Province, China. College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, Hebei Province, China. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Rural Energy and Environment Agency, Ministry of Agriculture and Rural Affairs, Beijing, China" |
| ISSN/ISBN: | 1744-7917 (Electronic) 1672-9609 (Linking) |
| Abstract: | "Semiochemical-based management strategies are important for controlling bark beetles, such as invasive Red Turpentine Beetle (Denroctonus valens), the causal agent for mass mortality of pine trees (Pinus spp.) in China. It has been previously shown that the pheromone verbenone regulates the attack density of this beetle in a dose-dependent manner and that the gut bacteria of D. valens are involved in verbenone production. However, molecular functional verification of the role of gut bacteria in the pheromone production of D. valens is still lacking. To better understand the molecular function of gut bacterial verbenone production, we chose a facultative anaerobic gut bacterium (Enterobacter xiangfangensis) of D. valens based on its strong ability to convert cis-verbenol to verbenone, as shown in our previous study, and investigated its transcriptomics in the presence or absence of cis-verbenol under anaerobic conditions (simulating the anoxic environment in the beetle's gut). Based on this transcriptome analysis, aldehyde dehydrogenase (ALDH1) was identified as a putative key gene responsible for verbenone production and was knocked-down by homologous recombination to obtain a mutant E. xiangfangensis strain. Our results show that these mutants had significantly decreased the ability to convert the monoterpene precursor to verbenone compared with the wild-type bacteria, indicating that ALDH1 is primarily responsible for verbenone conversion for this bacterium species. These findings provide further mechanistic evidence of bacterially mediated pheromone production by D. valens, add new perspective for functional studies of gut bacteria in general, and may aid the development of new gene silencing-based pest management strategies" |
| Keywords: | Animals *Coleoptera/physiology Aldehyde Dehydrogenase Pheromones Plant Bark Bacteria/genetics *Pinus Dendroctonus valens gut bacteria mutants transcriptome verbenone; |
| Notes: | "MedlineCao, Qingjie Koski, Tuuli-Marjaana Li, Huiping Zhang, Chi Sun, Jianghua eng Australia 2022/08/26 Insect Sci. 2023 Apr; 30(2):459-472. doi: 10.1111/1744-7917.13101. Epub 2022 Sep 13" |
