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Biochemistry

Title:		Protein structure encodes the ligand binding specificity in pheromone binding proteins
Author(s):		Du G; Prestwich GD;
Address:		"Department of Chemistry, University at Stony Brook, New York 11794-3400, USA"
Journal Title:		Biochemistry
Year:		1995
Volume:		34
Issue:		27
Page Number:		8726 - 8732
DOI:		10.1021/bi00027a023
ISSN/ISBN:		0006-2960 (Print) 0006-2960 (Linking)
Abstract:		"The ligand specificities and binding affinities of three recombinant pheromone binding proteins (PBPs) of two saturniid moths (genus Antheraea) were determined by using a novel binding assay in conjunction with two tritium-labeled constituents of the pheromone blend, [3H]-6E,11Z-hexadecadienyl acetate and [3H]-4E,9Z-tetradecadienyl acetate. The new binding assay, in which nonspecific adsorption to a plastic vessel is suppressed by presaturation of the surface with a 1-alkanol, allows measurement of dissociation constants (KD) for lipophilic ligands for their carrier proteins. The three PBPs showed KD values for [3H]-6E,11Z-16:Ac and [3H]-4E,9Z-14:Ac between 0.6 and 30 microM, as determined by Scatchard analysis. Importantly, two PBPs (Aper-1 and Aper-2) from one species showed opposite binding specificities for these two ligands. Aper-1, like Apol-3, showed 15-fold higher affinity for 6E,11Z-16:Ac than for 4E,9Z-14:Ac, while Aper-2 showed a 3.5-fold preference for binding the shorter chain compound. In addition, for the Apol-3 PBP, displacement of [3H]-6E,11Z-16:Ac binding by other pheromone components or analogs showed a clear trend in relative binding affinity: 6E,11Z-16:Ac > 4E,9Z-14:Ac > 6E,11Z-16:Al approximately 16:Ac > 6E,11Z-16:OH > 4E,9Z-14:OH. These data clearly demonstrate a > 1000-fold range of binding affinities among these very similar structures and unambiguously demonstrate the specificity of the PBP-pheromone interaction. Moreover, this assay offers the potential for determining ligand specificities for odorant binding proteins and other proteins in the vertebrate lipocalin superfamily"
Keywords:		Amino Acid Sequence Animals Base Sequence Carrier Proteins/chemistry/*metabolism Insect Hormones/chemistry/*metabolism *Insect Proteins Intercellular Signaling Peptides and Proteins Molecular Sequence Data Moths Oligodeoxyribonucleotides Protein Conformat;
Notes:		"MedlineDu, G Prestwich, G D eng NS 29632/NS/NINDS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1995/07/11 Biochemistry. 1995 Jul 11; 34(27):8726-32. doi: 10.1021/bi00027a023"