| Title: | RNAi-Induced Electrophysiological and Behavioral Changes Reveal two Pheromone Binding Proteins of Helicoverpa armigera Involved in the Perception of the Main Sex Pheromone Component Z11-16:Ald |
| Author(s): | Dong K; Sun L; Liu JT; Gu SH; Zhou JJ; Yang RN; Dhiloo KH; Gao XW; Guo YY; Zhang YJ; |
| Address: | "Department of Entomology, China Agricultural University, Beijing, 100193, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China. Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, AL5 2JQ, UK. Department of Entomology, Faculty of Crop Protection, Sindh Agriculture University Tandojam, Tandojam, Pakistan. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. yjzhang39@hotmail.com" |
| DOI: | 10.1007/s10886-016-0816-6 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "Pheromone binding proteins (PBPs) are thought to play key roles in insect sex pheromone recognition; however, there is little in vivo evidence to support this viewpoint in comparison to abundant biochemical data in vitro. In the present study, two noctuid PBP genes HarmPBP1 and HarmPBP2 of the serious agricultural pest, Helicoverpa armigera were selected to be knocked down by RNA interference, and then the changes in electrophysiological and behavioral responses of male mutants to their major sex pheromone component (Z)-11-hexadecenal (Z11-16:Ald) were recorded. There were no significant electrophysiological or behavioral changes of tested male moths in response to Z11-16:Ald when either single PBP gene was knocked down. However, decreased sensitivity of male moths in response to Z11-16:Ald was observed when both HarmPBP1 and HarmPBP2 genes were silenced. These results reveal that both HarmPBP1 and HarmPBP2 are required for the recognition of the main sex pheromone component Z11-16:Ald in H. armigera. Furthermore, these findings may help clarify physiological roles of moth PBPs in the sex pheromone recognition pathway, which in turn could facilitate pest control by exploring sex pheromone blocking agents" |
| Keywords: | "Animals Arthropod Antennae/drug effects/physiology Behavior, Animal/drug effects/*physiology Electrophysiological Phenomena Gene Knockdown Techniques Insect Control Insect Proteins/genetics/*metabolism Ketones/metabolism/*pharmacology Male *Moths/genetics;" |
| Notes: | "MedlineDong, Kun Sun, Liang Liu, Jing-Tao Gu, Shao-Hua Zhou, Jing-Jiang Yang, Ruo-Nan Dhiloo, Khalid Hussain Gao, Xi-Wu Guo, Yu-Yuan Zhang, Yong-Jun eng 2017/01/11 J Chem Ecol. 2017 Feb; 43(2):207-214. doi: 10.1007/s10886-016-0816-6. Epub 2017 Jan 9" |
