| Title: | A Terpene Synthase Is Involved in the Synthesis of the Volatile Organic Compound Sodorifen of Serratia plymuthica 4Rx13 |
| Author(s): | Domik D; Thurmer A; Weise T; Brandt W; Daniel R; Piechulla B; |
| Address: | "Institute for Biological Sciences, University of Rostock Rostock, Germany. Institute of Microbiology and Genetics, Applied Microbiology and Gottingen Genomics Laboratory, University of Gottingen Gottingen, Germany. Euroimmun AG Lubeck, Germany. Leibniz Institute of Plant Biochemistry Halle, Germany" |
| ISSN/ISBN: | 1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking) |
| Abstract: | "Bacteria release a plethora of volatile organic compounds, including compounds with extraordinary structures. Sodorifen (IUPAC name: 1,2,4,5,6,7,8-heptamethyl-3-methylenebicyclo[3.2.1]oct-6-ene) is a recently identified and unusual volatile hydrocarbon that is emitted by the rhizobacterium Serratia plymuthica 4Rx13. Sodorifen comprises a bicyclic ring structure solely consisting of carbon and hydrogen atoms, where every carbon atom of the skeleton is substituted with either a methyl or a methylene group. This unusual feature of sodorifen made a prediction of its biosynthetic origin very difficult and so far its biosynthesis is unknown. To unravel the biosynthetic pathway we performed genome and transcriptome analyses to identify candidate genes. One knockout mutant (SOD_c20750) showed the desired negative sodorifen phenotype. Here it was shown for the first time that this gene is indispensable for the synthesis of sodorifen and strongly supports the hypothesis that sodorifen descends from the terpene metabolism. SOD_c20750 is the first bacterial terpene cyclase isolated from Serratia spp. and Enterobacteriales. Homology modeling revealed a 3D structure, which exhibits a functional role of amino acids for intermediate cation stabilization (W325) and putative proton acception (Y332). Moreover, the size and hydrophobicity of the active site strongly indicates that indeed the enzyme may catalyze the unusual compound sodorifen" |
| Keywords: | Serratia bacterial terpene cyclase sodorifen volatile organic compound; |
| Notes: | "PubMed-not-MEDLINEDomik, Dajana Thurmer, Andrea Weise, Teresa Brandt, Wolfgang Daniel, Rolf Piechulla, Birgit eng Switzerland 2016/06/01 Front Microbiol. 2016 May 19; 7:737. doi: 10.3389/fmicb.2016.00737. eCollection 2016" |
