| Title: | Yeast KEX1 gene encodes a putative protease with a carboxypeptidase B-like function involved in killer toxin and alpha-factor precursor processing |
| Author(s): | Dmochowska A; Dignard D; Henning D; Thomas DY; Bussey H; |
| DOI: | 10.1016/0092-8674(87)90030-4 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "The yeast KEX1 gene product has homology to yeast carboxypeptidase Y. A mutant replacing serine at the putative active site of the KEX1 protein abolished activity in vivo. A probable site of processing by the KEX1 product is the C-terminus of the alpha-subunit of killer toxin, where toxin is followed in the precursor by 2 basic residues. Processing involves endoproteolysis following these basic residues and trimming of their C-terminal by a carboxypeptidase. Consistent with the KEX1 product being this carboxypeptidase is its role in alpha-factor pheromone production. In wild-type yeast, KEX1 is not essential for alpha-factor production, as the final pheromone repeat needs no C-terminal processing. However, in a mutant in which alpha-factor production requires a carboxypeptidase, pheromone production is KEX1-dependent" |
| Keywords: | "Amino Acid Sequence Base Sequence Carboxypeptidases/genetics Cathepsin A Fungal Proteins/*genetics/physiology *Genes, Fungal Killer Factors, Yeast Mating Factor *Peptide Biosynthesis *Peptides *Protein Biosynthesis Protein Precursors/metabolism *Protein P;" |
| Notes: | "MedlineDmochowska, A Dignard, D Henning, D Thomas, D Y Bussey, H eng Comparative Study Research Support, Non-U.S. Gov't 1987/08/14 Cell. 1987 Aug 14; 50(4):573-84. doi: 10.1016/0092-8674(87)90030-4" |
