| Title: | Pilot for Validation of Online Pretreatments for Analyses of Organics by Gas Chromatography-Mass Spectrometry: Application to Space Research |
| Author(s): | David M; Musadji NY; Labanowski J; Sternberg R; Geffroy-Rodier C; |
| Address: | "Universite Paris-Est-Creteil, Laboratoire Interuniversitaire des Systemes Atmospheriques (LISA), UMR CNRS 7583, 61 avenue du General de Gaulle, 94010 Creteil, France. Universite de Poitiers, Institut de Chimie des Milieux et Materiaux de Poitiers (IC2MP), UMR CNRS 7285, Equipe Eau Geochimie Sante, 4 rue Michel Brunet, 86073 Poitiers, France" |
| DOI: | 10.1021/acs.analchem.6b00052 |
| ISSN/ISBN: | 1520-6882 (Electronic) 0003-2700 (Linking) |
| Abstract: | "The search for complex organic molecules in extraterrestrial environments, including important biomolecules such as amino and fatty acids, will require a space compatible sample handling system to enable their detection by gas chromatography-mass spectrometry (GC-MS). For the future Mars exploratory mission Exomars 2018 aimed at organic molecules detection, a dedicated laboratory pilot, called Device for Pretreatment of Sample (DPS), reproducing representative space operating conditions has been developed. After its optimization, it aimed at validating under development protocols and interpreting forthcoming in situ resulting data. The DPS, dedicated to organic compounds' analysis, is discussed in terms of its technical features. The derivatization is carried out on a 50-100 mg mineral sample in a 4 mL reactor coupled with a GC-MS injector to simulate on line in situ derivatization-volatilization-transfer steps. Three derivatization reactions have been carried out with N-methyl-N-(tert-butyldimethylsilyl)-trifluoroacetamide (MTBSTFA) as silylating reagent, N,N-dimethylformamide dimethylacetal (DMF-DMA) and tetramethylammonium hydroxide (TMAH) as methylating agents. The performances are illustrated by comparison of conventional and in situ silylation, developed for space research applications, using terrestrial mineral matrix and Mars analog materials enriched with 25 nmol of each targeted organic molecule. The work presented in this rationale has established that the use of derivatization reactions widens the scope of targeted molecules but also clearly points out mineral matrix effect. Decreasing mineral influence on pretreatment will be the next scientific challenge in in situ analysis" |
| Notes: | "PubMed-not-MEDLINEDavid, M Musadji, N-Y Labanowski, J Sternberg, R Geffroy-Rodier, C eng Research Support, Non-U.S. Gov't 2016/04/26 Anal Chem. 2016 May 17; 88(10):5137-44. doi: 10.1021/acs.analchem.6b00052. Epub 2016 May 2" |
