| Title: | A receptor-neuron correlate for the detection of attractive plant volatiles in Helicoverpa assulta (Lepidoptera: Noctuidae) |
| Author(s): | Cui WC; Wang B; Guo MB; Liu Y; Jacquin-Joly E; Yan SC; Wang GR; |
| Address: | "College of Forestry, Northeast Forestry University, 150040 Harbin, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193 Beijing, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193 Beijing, China. INRA, Institute of Ecology & Environmental Sciences of Paris, Versailles, France. College of Forestry, Northeast Forestry University, 150040 Harbin, China. Electronic address: yanshanchun@126.com. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193 Beijing, China. Electronic address: wangguirong@caas.cn" |
| DOI: | 10.1016/j.ibmb.2018.04.006 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "Plant volatiles are vital cues in the location of hosts for feeding and oviposition for Lepidoptera moths. The noctuid Helicoverpa assulta is a typical polyphagous moth, regarded as a good model for studying the olfactory reception of plant volatiles. In this study, four full-length genes encoding odorant receptors HassOR24, HassOR40, HassOR41, and HassOR55 expressed in antenna in H. assulta were functionally characterized. The highly expressed HassOR40 was narrowly tuned to a few structurally-related plant volatiles: geranyl acetate, geraniol and nerolidol. By systematically analyzing responses of single neuron in both trichoid sensilla and basiconic sensilla using single sensillum recording, the specific neuron B in one type of short trichoid sensilla was found to be mainly activated by the same chemicals as HassOR40 with high sensitivity, and with no significant difference between male and female neurons. Thus, a clear 'receptor-neuron' relationship in H. assulta was demonstrated here, suggesting that HassOR40/HassOrco are expressed in neuron B of short trichoid sensilla. The active tobacco volatile nerolidol, recognized by this receptor-neuron line, elicits significant behavioral attraction of both sexes in H. assulta adults. The results indicate that we identified a receptor-neuron route for the peripheral coding of a behaviorally relevant host volatile in H. assulta" |
| Keywords: | "Animals Arthropod Antennae/cytology/innervation/*metabolism Gene Expression Regulation/physiology Insect Proteins/*biosynthesis/genetics Lepidoptera/cytology/genetics/*metabolism Neurons/cytology/*metabolism Receptors, Odorant/*biosynthesis/genetics Volat;" |
| Notes: | "MedlineCui, Wei-Chan Wang, Bing Guo, Meng-Bo Liu, Yang Jacquin-Joly, Emmanuelle Yan, Shan-Chun Wang, Gui-Rong eng Research Support, Non-U.S. Gov't England 2018/04/27 Insect Biochem Mol Biol. 2018 Jun; 97:31-39. doi: 10.1016/j.ibmb.2018.04.006. Epub 2018 Apr 23" |
