| Title: | TPS Genes Silencing Alters Constitutive Indirect and Direct Defense in Tomato |
| Author(s): | Coppola M; Cascone P; Bossi S; Corrado G; Garonna AP; Maffei M; Rao R; Guerrieri E; |
| Address: | "Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy. mariangela.coppola@unina.it. Institute for Sustainable Plant Protection, National Research Council of Italy, 80055 Portici (Na), Italy. pasquale.cascone@ipsp.cnr.it. Department of Life Sciences and Systems Biology, University of Turin, 10135 Turin, Italy. simone.bossi@unito.it. Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy. giacorra@unina.it. Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy. antoniopietro.garonna@unina.it. Department of Life Sciences and Systems Biology, University of Turin, 10135 Turin, Italy. massimo.maffei@unito.it. Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy. rosa.rao@unina.it. Institute for Sustainable Plant Protection, National Research Council of Italy, 80055 Portici (Na), Italy. emilio.guerrieri@ipsp.cnr.it" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Following herbivore attacks, plants modify a blend of volatiles organic compounds (VOCs) released, resulting in the attraction of their antagonists. However, volatiles released constitutively may affect herbivores and natural enemies' fitness too. In tomato there is still a lack of information on the genetic bases responsible for the constitutive release of VOC involved in direct and indirect defenses. Here we studied the constitutive emissions related to the two most abundant sesquiterpene synthase genes expressed in tomato and their functional role in plant defense. Using an RNA interference approach, we silenced the expression of TPS9 and TPS12 genes and assessed the effect of this transformation on herbivores and parasitoids. We found that silenced plants displayed a different constitutive volatiles emission from controls, resulting in reduced attractiveness for the aphid parasitoid Aphidius ervi and in an impaired development of Spodoptera exigua larvae. We discussed these data considering the transcriptional regulation of key-genes involved in the pathway of VOC metabolism. We provide several lines of evidence on the metabolic flux from terpenoids to phenylpropanoids. Our results shed more light on constitutive defenses mediated by plant volatiles and on the molecular mechanisms involved in their metabolic regulation" |
| Keywords: | Animals Aphids/physiology Herbivory/*physiology Host-Parasite Interactions Solanum lycopersicum/*metabolism/*parasitology Spodoptera/physiology Volatile Organic Compounds/metabolism Wasps/physiology Aphidius ervi RNAi Sesquiterpene Synthase Spodoptera exi; |
| Notes: | "MedlineCoppola, Mariangela Cascone, Pasquale Bossi, Simone Corrado, Giandomenico Garonna, Antonio Pietro Maffei, Massimo Rao, Rosa Guerrieri, Emilio eng Switzerland 2018/09/16 Int J Mol Sci. 2018 Sep 13; 19(9):2748. doi: 10.3390/ijms19092748" |
