| Title: | Pheromone-induced phosphorylation of a G protein beta subunit in S. cerevisiae is associated with an adaptive response to mating pheromone |
| Address: | "Molecular Biology Department, Research Institute of Scripps Clinic, La Jolla, California 92037" |
| DOI: | 10.1016/0092-8674(91)90500-x |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "The mating pheromone response in S. cerevisiae is activated by a G protein-mediated signaling pathway in which G beta gamma is the active transducer of the signal. When exogenous pheromone is added to vegetatively growing cells, G beta is rapidly phosphorylated at several sites; phosphorylation does not require de novo protein synthesis. A mutation in G beta was constructed that eliminates signal-induced phosphorylation. This mutation leads to enhanced sensitivity to and impaired ability to recover from pheromone, but does not affect the ability of G beta gamma to transmit the mating signal. These phenotypes suggest that G protein phosphorylation mediates an adaptive response to pheromone-induced signaling. G beta phosphorylation does not require either the pheromone receptor C-terminus or the product of the SST2 gene, both of which mediate separate adaptive responses to pheromone. However, G beta phosphorylation is greatly facilitated by the presence of the G alpha subunit, which has also been shown to participate in an adaptation to pheromone" |
| Keywords: | "Amino Acid Sequence Amino Acids/analysis Chromosome Deletion Crosses, Genetic *GTP-Binding Protein beta Subunits GTP-Binding Proteins/genetics/*metabolism Genes, Fungal Genotype *Heterotrimeric GTP-Binding Proteins Macromolecular Substances Mating Factor;" |
| Notes: | "MedlineCole, G M Reed, S I eng GM 13116/GM/NIGMS NIH HHS/ GM49429/GM/NIGMS NIH HHS/ Comparative Study Research Support, U.S. Gov't, P.H.S. 1991/02/22 Cell. 1991 Feb 22; 64(4):703-16. doi: 10.1016/0092-8674(91)90500-x" |
