| Title: | Understanding the Functional Impact of VOC-Ozone Mixtures on the Chemistry of RNA in Epithelial Lung Cells |
| Author(s): | Contreras LM; Gonzalez-Rivera JC; Baldridge KC; Wang DS; Chuvalo-Abraham J; Ruiz LH; |
| Address: | "McKetta Department of Chemical Engineering, University of Texas, Austin" |
| ISSN/ISBN: | 1041-5505 (Print) 2688-6855 (Electronic) 1041-5505 (Linking) |
| Abstract: | "INTRODUCTION: Ambient air pollution is associated with premature death caused by heart disease, stroke, chronic obstructive pulmonary disease (COPD), and lung cancer. Recent studies have suggested that ribonucleic acid (RNA) oxidation is a sensitive environment-related biomarker that is implicated in pathogenesis. AIMS AND METHODS: We used a novel approach that integrated RNA-Seq analysis with detection by immunoprecipitation techniques of the prominent RNA oxidative modification 8-oxo-7,8-dihydroguanine (8-oxoG). Our goal was to uncover specific messenger RNA (mRNA) oxidation induced by mixtures of volatile organic compounds (VOCs) and ozone in healthy human epithelial lung cells. To this end, we exposed the BEAS-2B human epithelial lung cell line to the gas- and particle-phase products formed from reactions of 790 ppb acrolein (ACR) and 670 ppb methacrolein (MACR) with 4 ppm ozone. RESULTS: Using this approach, we identified 222 potential direct targets of oxidation belonging to previously described pathways, as well as uncharacterized pathways, after air pollution exposures. We demonstrated the effect of our VOC-ozone mixtures on the morphology and actin cytoskeleton of lung cells, suggesting the influence of selective mRNA oxidation in members of pathways regulating physical components of the cells. In addition, we observed the influence of the VOC-ozone mixtures on metabolic cholesterol synthesis, likely implicated as a result of the incidence of mRNA oxidation and the deregulation of protein levels of squalene synthase (farnesyl-diphosphate farnesyltransferase 1 [FDFT1]), a key enzyme in endogenous cholesterol biosynthesis. CONCLUSIONS: Overall, our findings indicate that air pollution influences the accumulation of 8-oxoG in transcripts of epithelial lung cells that largely belong to stress-induced signaling and metabolic and structural pathways. A strength of the study was that it combined traditional transcriptome analysis with transcriptome-wide 8-oxoG mapping to facilitate the discovery of underlying processes not characterized by earlier approaches. Investigation of the processes mediated by air pollution oxidation of RNA molecules in primary cells and animal models needs to be explored in future studies. Our research has thus opened new avenues to further inform the relationship between atmospheric agents on the one hand and cellular responses on the other that are implicated in diseases" |
| Keywords: | Acrolein/analogs & derivatives/pharmacology Air Pollutants/*pharmacology Down-Regulation Epithelial Cells/*drug effects Humans Lung/*drug effects Oxidation-Reduction Ozone/*pharmacology RNA/*drug effects Time Factors Volatile Organic Compounds/*pharmacolo; |
| Notes: | "MedlineContreras, L M Gonzalez-Rivera, J C Baldridge, K C Wang, D S Chuvalo-Abraham, Jcl Ruiz, L H eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Technical Report 2020/08/28 Res Rep Health Eff Inst. 2020 Jul; 2020(201):201" |
