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Mol Cell Biol

Title:		The yeast Mcm1 protein is regulated posttranscriptionally by the flux of glycolysis
Author(s):		Chen Y; Tye BK;
Address:		"Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA"
Journal Title:		Mol Cell Biol
Year:		1995
Volume:		15
Issue:		8
Page Number:		4631 - 4639
DOI:		10.1128/MCB.15.8.4631
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Mcm1 is a multifunctional protein which plays a role both in the initiation of DNA replication and in the transcriptional regulation of diverse genes in Saccharomyces cerevisiae. The mcm1-1 mutation results in instability of minichromosomes and alpha-specific sterility. Second-site suppressors that restore minichromosome stability but not fertility to the mcm1-1 mutant were isolated. Two of the suppressors, pgm1-1 and pgm1-2, are mutant alleles of PGM1 which encodes a glycolytic enzyme, phosphoglycerate mutase. We show that the pgm1-1 mutation suppresses the minichromosome maintenance (Mcm) defect by increasing the protein activity or level of Mcm1-1 posttranscriptionally. This increase in the intracellular Mcm1-1 activity is sufficient to suppress the Mcm defect but only minimally suppresses the mating defect. Mutations in genes encoding other glycolytic enzymes, such as eno2::URA3, can also suppress the Mcm phenotype of mcm1-1. Suppression by these glycolytic enzyme mutations correlates with a reduced rate of glycolysis rather than a reduced rate of cell growth. This study suggests that in response to changes in their nutritional states yeast cells may attain homeostasis by modulating the activity of global regulators like Mcm1, which plays a central role in the regulation of energy-expensive anabolic processes"
Keywords:		"Alleles Cell Compartmentation Chromosomes, Fungal/genetics DNA Replication/genetics DNA-Binding Proteins/*genetics Energy Metabolism/physiology Ethanol/metabolism Fungal Proteins/genetics *Gene Expression Regulation, Fungal Genes, Fungal/genetics Genes, R;"
Notes:		"MedlineChen, Y Tye, B K eng GM34190/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1995/08/01 Mol Cell Biol. 1995 Aug; 15(8):4631-9. doi: 10.1128/MCB.15.8.4631"