| Title: | Olfactory dysfunction and potential mechanisms caused by volatile organophosphate dichlorvos in the silkworm as a model animal |
| Author(s): | Chen J; Li SS; Fang SM; Zhang Z; Yu QY; |
| Address: | "Laboratory of Evolutionary and Functional Genomics, School of Life Sciences, Chongqing University, Chongqing 400044, China. College of Life Science, China West Normal University, Nanchong 637002, Sichuan, China. Laboratory of Evolutionary and Functional Genomics, School of Life Sciences, Chongqing University, Chongqing 400044, China. Electronic address: yuqy@cqu.edu.cn" |
| DOI: | 10.1016/j.jhazmat.2021.127940 |
| ISSN/ISBN: | 1873-3336 (Electronic) 0304-3894 (Linking) |
| Abstract: | "Volatile pesticides impair olfactory function in workers/farmers and insects, but data on molecular responses and mechanisms are poorly understood. This study aims to reveal the mechanisms of olfactory dysfunction in the silkworm after exposure to volatile dichlorvos. Our results demonstrated that acute exposure for 12 h significantly reduced electroantennogram responses, and over 62.50% of the treated male moths cannot locate the pheromone source. Transcriptional and proteomic responses of the antennae and heads were investigated. A total of 101 differentially expressed genes (DEGs) in the antennae, 138 DEGs in the heads, and 43 differentially expressed proteins (DEPs) in the heads including antennae were revealed. We discovered that upregulations of Arrestin1 and nitric oxide synthase1 (NOS1) may inhibit cyclic nucleotide-gated channels and hinder calcium influx in the antennae. In the central nervous systems (CNS), downregulations of tyrosine hydroxylase (TH) and tyrosine decarboxylase (TDC) may inhibit olfactory signal transduction by reducing the second messenger biosynthesis. Meanwhile, an abnormal increase of brain cell apoptosis was revealed by Annexin V-mCherry staining, often leading to persistent neurologic impairment. Taken together, this study highlighted olfactory dysfunction caused by dichlorvos, which may provide a novel perspective for understanding the toxicity mechanism of volatile pesticides in other organisms" |
| Keywords: | Animals *Bombyx/genetics Dichlorvos/toxicity Humans Male *Olfaction Disorders Organophosphates Proteomics Courtship behavior Olfactory dysfunction Silkworm Transcriptional and proteomic responses volatile dichlorvos; |
| Notes: | "MedlineChen, Jie Li, Shu-Shang Fang, Shou-Min Zhang, Ze Yu, Quan-You eng Research Support, Non-U.S. Gov't Netherlands 2021/12/14 J Hazard Mater. 2022 Mar 5; 425:127940. doi: 10.1016/j.jhazmat.2021.127940. Epub 2021 Nov 28" |
