| Title: | Ribonuclease1 contributes to the antibacterial response and immune defense in blunt snout bream (Megalobrama amblycephala) |
| Author(s): | Chen J; Huang X; Geng R; Zhu D; Wang W; Liu H; |
| Address: | "College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: wangwm@mail.hzau.edu.cn. College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: liuhan@mail.hzau.edu.cn" |
| DOI: | 10.1016/j.ijbiomac.2021.01.066 |
| ISSN/ISBN: | 1879-0003 (Electronic) 0141-8130 (Linking) |
| Abstract: | "Ribonuclease 1 (RNase1) is a vertebrate-specific enzyme that mainly performs digestive activity in herbivorous mammals. Here we used bacterial viability assays to explore its antimicrobial activity in blunt snout bream (Megalobrama amblycephala). The results showed that Ma-RNase1 rapidly killed Gram-negative and Gram-positive bacteria at micromolar concentrations. Ma-RNase1 increased the permeability of bacterial outer and inner membranes, thus reducing the integrity of bacterial cell wall and membrane. Moreover, Ma-RNase1 effectively counteracted the tissue damage and apoptosis caused by Aeromonas hydrophila infection. Quantitative real-time PCR and immunoblot analysis indicated that RNase1 mRNA and protein were up-regulated in the kidney and gut during infection. Furthermore, A. hydrophila infection significantly induced Tnf-alpha and Il-1beta mRNA expression in liver, but not in the RNase1 pre-treatment group. In addition, a significant increase in the expression of immune-related genes (Nf-kappab and Tlr4) was found in liver, kidney and gut of A. hydrophila-infected fish, while a decrease in Myd88 and Tlr4 levels was found in liver, spleen, kidney and gut in the group pre-treated with RNase1. Collectively, these data suggest that Ma-RNase1 has antimicrobial function both in vitro and in vivo, and contributes to the protective effect and immune defense of blunt snout bream" |
| Keywords: | Aeromonas hydrophila/growth & development/*immunology/pathogenicity Animals Cell Membrane/immunology/metabolism Cell Membrane Permeability Cyprinidae/*genetics/immunology/microbiology Disease Resistance/genetics/immunology Fish Diseases/enzymology/*geneti; |
| Notes: | "MedlineChen, Jing Huang, Xin Geng, Ruijing Zhu, Dongmei Wang, Weimin Liu, Han eng Netherlands 2021/01/18 Int J Biol Macromol. 2021 Mar 1; 172:309-320. doi: 10.1016/j.ijbiomac.2021.01.066. Epub 2021 Jan 14" |
