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J Plant Physiol

Title:		Protein profiling and tps23 induction in different maize lines in response to methyl jasmonate treatment and Diabrotica virgifera infestation
Author(s):		Capra E; Colombi C; De Poli P; Nocito FF; Cocucci M; Vecchietti A; Marocco A; Stile MR; Rossini L;
Address:		"Istituto di Biologia e Biotecnologia Agraria Consiglio Nazionale delle Ricerche IBBA-CNR, via Einstein, 26900 Lodi, Italy; Biotrack S.r.l., Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy. Electronic address: e.capra@ibba.cnr.it. Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy. Biotrack S.r.l., Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy. Universita degli Studi di Milano, DiSAA, via Celoria 2, 20133 Milan, Italy. Istituto di Agronomia, Genetica e Coltivazioni erbacee, Universita Cattolica del Sacro Cuore, via Emilia Parmense 84, 29122 Piacenza, Italy. Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy; Agroalimentare Sud SpA, Loc. S. Nicola, 85025 Melfi, PZ, Italy. Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy; Universita degli Studi di Milano, DiSAA, via Celoria 2, 20133 Milan, Italy"
Journal Title:		J Plant Physiol
Year:		2015
Volume:		20141127
Issue:
Page Number:		68 - 77
DOI:		10.1016/j.jplph.2014.10.018
ISSN/ISBN:		1618-1328 (Electronic) 0176-1617 (Linking)
Abstract:		"Plant responses to herbivore insects involve direct and indirect defense with the production of signal molecules including jasmonic acid (JA) and its derivatives (e.g. methyl jasmonate, MeJA). In maize (Zea mays), root feeding by Diabrotica virgifera larvae activates an indirect defense mechanism, through enthomopathogenic nematodes that are recruited after Terpene Synthase 23 (tps23) upregulation and (E)-beta-caryophyllene root emission. In order to gain insight into the correlation between JA signaling and response to Diabrotica attack, we analyzed tps23 expression and protein profiles in maize roots in response to MeJA treatment and insect infestation. Similar to herbivore feeding, MeJA treatment was found to increase tps23 transcript accumulation, with consistent variations for both treatments in maize lines differing in (E)-beta-caryophyllene production. Analysis of root protein profiles showed specific alterations leading to the identification of three proteins that were induced by MeJA treatment. We focused on a peroxidase-like protein (Px-like) showing that the corresponding transcripts accumulated in all tested lines. Results show that exogenous application of MeJA upregulates tps23 expression and specifically alters protein patterns in maize roots. Parallel effects on tps23 transcript accumulation were observed upon hormone exposure and insect infestation in different maize lines. In contrast, Px-like transcript profiling showed differences between treatments. These results support the possible involvement of MeJA in mediating the upregulation of tps23 in response to Diabrotica attack"
Keywords:		"Acetates/*pharmacology Alkyl and Aryl Transferases/genetics/metabolism Animals Coleoptera/*physiology Cyclopentanes/*pharmacology Gene Expression Regulation, Plant Herbivory Larva Oxylipins/*pharmacology Plant Growth Regulators/*pharmacology Plant Protein;"
Notes:		"MedlineCapra, Emanuele Colombi, Cinzia De Poli, Pamela Nocito, Fabio Francesco Cocucci, Maurizio Vecchietti, Alberto Marocco, Adriano Stile, Maria Rosaria Rossini, Laura eng Research Support, Non-U.S. Gov't Germany 2014/12/17 J Plant Physiol. 2015 Mar 1; 175:68-77. doi: 10.1016/j.jplph.2014.10.018. Epub 2014 Nov 27"