« Previous AbstractResearch progress in the enhancement technology of soil vapor extraction of volatile petroleum hydrocarbon pollutants    Next AbstractTranscriptional and epigenetic analysis reveals that NAC transcription factors regulate fruit flavor ester biosynthesis »

J Agric Food Chem

Title:		Peach Carboxylesterase PpCXE1 Is Associated with Catabolism of Volatile Esters
Author(s):		Cao X; Xie K; Duan W; Zhu Y; Liu M; Chen K; Klee H; Zhang B;
Address:		"Laboratory of Fruit Quality Biology/Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology , Zhejiang University , Zijingang Campus , Hangzhou 310058 , China. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences , Sichuan University , Chengdu 610065 , China. Horticultural Sciences, Plant Innovation Center, Genetic Institute , University of Florida , Gainesville , Florida 32611 , United States"
Journal Title:		J Agric Food Chem
Year:		2019
Volume:		20190424
Issue:		18
Page Number:		5189 - 5196
DOI:		10.1021/acs.jafc.9b01166
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Peach fruit volatile acetate esters impact consumer sensory preference and contribute to defense against biotic stresses. Previous studies showed that alcohol acyltransferase (AAT) family PpAAT1 is correlated with volatile ester formation in peach fruits. However, fruits also contain carboxylesterase (CXE) enzymes that hydrolyze esters. The functions of this family with regard to volatile ester content has not been explored. Here, we observed that content of acetate ester was negatively correlated with expression of PpCXE1. Recombinant PpCXE1 protein exhibited hydrolytic activity toward acetate esters present in peach fruit. Kinetic analysis showed that PpCXE1 showed the highest catalytic activity toward E-2-hexenyl acetate. Subcellular localization demonstrated that PpCXE1 is present in the cytoplasm. Transient expression in peach fruit and stable overexpression in tomato fruit resulted in significant reduction of volatile esters in vivo. Taken together, the results indicate that PpCXE1 expression is associated with catabolism of volatile acetate esters in peach fruit"
Keywords:		Carboxylesterase/chemistry/genetics/*metabolism Esters/analysis/*metabolism Fruit/chemistry/enzymology/genetics Kinetics Plant Proteins/chemistry/genetics/*metabolism Prunus persica/chemistry/*enzymology/genetics Volatile Organic Compounds/analysis/*metab;
Notes:		"MedlineCao, Xiangmei Xie, Kaili Duan, Wenyi Zhu, Yunqi Liu, Mingchun Chen, Kunsong Klee, Harry Zhang, Bo eng 2019/04/19 J Agric Food Chem. 2019 May 8; 67(18):5189-5196. doi: 10.1021/acs.jafc.9b01166. Epub 2019 Apr 24"