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Mol Gen Mikrobiol Virusol

Title:		[Preparation of Saccharomyces cerevisiae strains with regulated MFalpha1 promotor activity]
Author(s):		Brutman AV; Ostanin KV; Miasnikov AN; Smirnov MN;
Address:
Journal Title:		Mol Gen Mikrobiol Virusol
Year:		1991
Volume:
Issue:		12
Page Number:		14 - 19
DOI:
ISSN/ISBN:		0208-0613 (Print) 0208-0613 (Linking)
Abstract:		"Two Saccharomyces cerevisiae strains containing integrated copies of the MAT alpha 1 gene fused to the PHO5 promoter have been obtained by transformation of a MAT alpha 1 mutant. The strains differ in length of 5'-uncoding region of the MAT alpha 1 in the integrated constructions. The mating activity and the ability of these strains to express the yeast MF alpha 1 gene and the hyman alpha-N-interferon gene under the control of MF alpha 1 promoter was shown to be regulated by the exogenous inorganic phosphate. The level of intracellular alpha-N-interferon synthesized in these strains was several fold higher as compared with the wild type alpha mating type strain. At the same time the observed increase in intracellular production is not accompanied by an increase in the level of secreted alpha-N-interferon. On the contrary, one of the strains had a two-fold reduction in the rate of secretion"
Keywords:		"Escherichia coli/metabolism Gene Expression Regulation, Fungal Genes, Fungal Genes, Mating Type, Fungal Interferon-alpha/genetics Mating Factor Peptides/metabolism Phosphates/metabolism Plasmids *Promoter Regions, Genetic Saccharomyces cerevisiae/*genetic;"
Notes:		"MedlineBrutman, A V Ostanin, K V Miasnikov, A N Smirnov, M N rus Russia (Federation) 1991/12/01 Mol Gen Mikrobiol Virusol. 1991 Dec; (12):14-9"