| Title: | "Persistent loss of hippocampal neurogenesis and increased cell death following adolescent, but not adult, chronic ethanol exposure" |
| Author(s): | Broadwater MA; Liu W; Crews FT; Spear LP; |
| Address: | "Bowles Center for Alcohol Studies, University of North Carolina at Chapel Hill, Chapel Hill, N.C., USA" |
| ISSN/ISBN: | 1421-9859 (Electronic) 0378-5866 (Print) 0378-5866 (Linking) |
| Abstract: | "Although adolescence is a common age to initiate alcohol consumption, the long-term consequences of exposure to alcohol at this time of considerable brain maturation are largely unknown. In studies utilizing rodents, behavioral evidence is beginning to emerge suggesting that the hippocampus may be persistently affected by repeated ethanol exposure during adolescence, but not by comparable alcohol exposure in adulthood. The purpose of this series of experiments was to explore a potential mechanism of hippocampal dysfunction in adults exposed to ethanol during adolescence. Given that disruption in adult neurogenesis has been reported to impair performance on tasks thought to be hippocampally related, we used immunohistochemistry to assess levels of doublecortin (DCX), an endogenous marker of immature neurons, in the dentate gyrus (DG) of the hippocampus 3-4 weeks after adolescent (postnatal day, PD28-48) or adult (PD70-90) intermittent ethanol exposure to 4 g/kg ethanol administered intragastrically. We also investigated another neurogenic niche, the subventricular zone (SVZ), to determine if the effects of ethanol exposure were region specific. Levels of cell proliferation and cell death were also examined in the DG via assessing Ki67 and cleaved caspase-3 immunoreactivity, respectively. Significantly less DCX was observed in the DG of adolescent (but not adult) ethanol-exposed animals about 4 weeks after exposure when these animals were compared to control age-mates. The effects of adolescent ethanol on DCX immunoreactivity were specific to the hippocampus, with no significant exposure effects emerging in the SVZ. In both the DG and the SVZ there was a significant age-related decline in neurogenesis as indexed by DCX. The persistent effect of adolescent ethanol exposure on reduced DCX in the DG appears to be related to significant increases in cell death, with significantly more cleaved caspase-3-positive immunoreactivity observed in the adolescent ethanol group compared to controls, but no alterations in cell proliferation when indexed by Ki67. These results suggest that a history of adolescent ethanol exposure results in lowered levels of differentiating neurons, probably due at least in part to increased cell death of immature neurons. These effects were evident in adulthood, weeks following termination of the chronic exposure, and may contribute to previously reported behavioral deficits on hippocampal-related tasks after chronic ethanol exposure in adolescence" |
| Keywords: | Aging/*physiology Animals Body Weight/drug effects Caspase 3/metabolism Cell Death/*drug effects Central Nervous System Depressants/*toxicity Dentate Gyrus/drug effects/growth & development Doublecortin Domain Proteins Doublecortin Protein Ethanol/*toxici; |
| Notes: | "MedlineBroadwater, Margaret A Liu, Wen Crews, Fulton T Spear, Linda P eng AA007573/AA/NIAAA NIH HHS/ T32 AA007573/AA/NIAAA NIH HHS/ U24 AA020024/AA/NIAAA NIH HHS/ AA020023/AA/NIAAA NIH HHS/ AA020022/AA/NIAAA NIH HHS/ U01 AA019972/AA/NIAAA NIH HHS/ AA019972/AA/NIAAA NIH HHS/ U24 AA020022/AA/NIAAA NIH HHS/ AA020024/AA/NIAAA NIH HHS/ U01 AA020023/AA/NIAAA NIH HHS/ Research Support, N.I.H., Extramural Switzerland 2014/07/06 Dev Neurosci. 2014; 36(3-4):297-305. doi: 10.1159/000362874. Epub 2014 Jun 27" |
