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J Cell Biol

Title:		Rvs161p interacts with Fus2p to promote cell fusion in Saccharomyces cerevisiae
Author(s):		Brizzio V; Gammie AE; Rose MD;
Address:		"Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA"
Journal Title:		J Cell Biol
Year:		1998
Volume:		141
Issue:		3
Page Number:		567 - 584
DOI:		10.1083/jcb.141.3.567
ISSN/ISBN:		0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking)
Abstract:		"FUS7 was previously identified by a mutation that causes a defect in cell fusion in a screen for bilateral mating defects. Here we show that FUS7 is allelic to RVS161/END6, a gene implicated in a variety of processes including viability after starvation, endocytosis, and actin cytoskeletal organization. Two lines of evidence indicate that RVS161/END6's endocytic function is not required for cell fusion. First, several other endocytic mutants showed no cell fusion defects. Second, we isolated five function-specific alleles of RVS161/FUS7 that were defective for endocytosis, but not mating, and three alleles that were defective for cell fusion but not endocytosis. The organization of the actin cytoskeleton was normal in the cell fusion mutants, indicating that Rvs161p's function in cell fusion is independent of actin organization. The three to fourfold induction of RVS161 by mating pheromone and the localization of Rvs161p-GFP to the cell fusion zone suggested that Rvs161p plays a direct role in cell fusion. The phenotypes of double mutants, the coprecipitation of Rvs161p and Fus2p, and the fact that the stability of Fus2p was strongly dependent on Rvs161p's mating function lead to the conclusion that Rvs161p is required to interact with Fus2p for efficient cell fusion"
Keywords:		Actins/metabolism Alleles Cytoskeletal Proteins/genetics/*metabolism Endocytosis Fungal Proteins/genetics/*metabolism/physiology Gene Deletion Gene Expression Mating Factor Membrane Fusion Membrane Proteins/genetics/*metabolism Peptides/physiology Sacchar;
Notes:		"MedlineBrizzio, V Gammie, A E Rose, M D eng R01 GM037739/GM/NIGMS NIH HHS/ GM37739/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1998/06/13 J Cell Biol. 1998 May 4; 141(3):567-84. doi: 10.1083/jcb.141.3.567"