« Previous AbstractParasitoids and dipteran predators exploit volatiles from microbial symbionts to locate bark beetles    Next AbstractRole of the vomeronasal organ in neonatal offspring recognition in sheep »

Biotechnol Lett

Title:		A rapid column technique for trapping and collecting of volatile fungal hydrocarbons and hydrocarbon derivatives
Author(s):		Booth E; Strobel G; Knighton B; Sears J; Geary B; Avci R;
Address:		"Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA"
Journal Title:		Biotechnol Lett
Year:		2011
Volume:		20110610
Issue:		10
Page Number:		1963 - 1972
DOI:		10.1007/s10529-011-0660-2
ISSN/ISBN:		1573-6776 (Electronic) 0141-5492 (Linking)
Abstract:		"A custom-made stainless steel column was designed to contain various materials that would trap the hydrocarbons and hydrocarbon derivatives during the processes of fungal fermentation ultimately yielding preparative amounts of volatile organic substances (VOCs). Trapping materials tested in the column were Carbotrap materials A and B (Supelco) as well as bentonite-shale from the oil bearing areas of Eastern Montana, the former allowed for the effective and efficient trapping of VOCs from purged cultures of Hypoxylon sp. Trapping efficiencies of various materials were measured by both gravimetric as well as proton transfer reaction mass spectroscopy with the Carbotraps A and B being 99% efficient when tested with known amounts of 1,8-cineole. Trapped fungal VOCs could effectively be removed and recovered via controlled heating of the stainless steel column followed by passage of the gases through a liquid nitrogen trap at a recovery rate of ca 65-70%. This method provides for the recovery of mg quantities of compounds normally present in the gas phase that may be needed for spectroscopy, bioassays and further separation and analysis and may have wide applicability for many other biological systems involving VOCs. Other available Carbotraps could be used for other applications"
Keywords:		"Bentonite Cyclohexanols Endophytes Equipment Design Eucalyptol Gas Chromatography-Mass Spectrometry/instrumentation/*methods Gases/analysis Hydrocarbons/chemistry/*isolation & purification Microscopy, Electron, Scanning Monoterpenes Volatile Organic Compo;"
Notes:		"MedlineBooth, Eric Strobel, Gary Knighton, Berk Sears, Joe Geary, Brad Avci, Recep eng Research Support, U.S. Gov't, Non-P.H.S. Netherlands 2011/06/11 Biotechnol Lett. 2011 Oct; 33(10):1963-72. doi: 10.1007/s10529-011-0660-2. Epub 2011 Jun 10"