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Plant Cell

Title:		"A Promiscuous CYP706A3 Reduces Terpene Volatile Emission from Arabidopsis Flowers, Affecting Florivores and the Floral Microbiome"
Author(s):		Boachon B; Burdloff Y; Ruan JX; Rojo R; Junker RR; Vincent B; Nicole F; Bringel F; Lesot A; Henry L; Bassard JE; Mathieu S; Allouche L; Kaplan I; Dudareva N; Vuilleumier S; Miesch L; Andre F; Navrot N; Chen XY; Werck-Reichhart D;
Address:		"Institut de Biologie Moleculaire des Plantes du Centre National de la Recherche Scientifique (CNRS), Unite Propre de Recherche 2357, Universite de Strasbourg, 67084 Strasbourg, France werck@unistra.fr. Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907. University of Lyon, UJM-Saint-Etienne, CNRS, BVpam Formation de Recherche en Evolution 3727, 42000 Saint-Etienne, France. Institut de Biologie Moleculaire des Plantes du Centre National de la Recherche Scientifique (CNRS), Unite Propre de Recherche 2357, Universite de Strasbourg, 67084 Strasbourg, France. National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, University of CAS, Chinese Academy of Sciences, Shanghai 200032, China. Plant Science Research Center, Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, China. Institute for Integrative Biology of the Cell (I2BC), iBiTec-S/SBSM, Commissariat a l'Energie Atomique, CNRS, Universite Paris Sud, Universite Paris-Saclay, 91191 Gif-sur-Yvette, France. Evolutionary Ecology of Plants, Department Biodiversity of Plants, Faculty of Biology, Philipps-University Marburg, 35043 Marburg, Germany. Plateforme d'Analyses pour la Chimie, GDS 3648, CNRS, Universite de Strasbourg, 67000 Strasbourg, France. Genetique Moleculaire, Genomique, Microbiologie, Universite de Strasbourg, UMR 7156 CNRS, 67000 Strasbourg, France. Department of Entomology, Purdue University, West Lafayette, Indiana 47907. Equipe de Synthese Organique et Phytochimie, Institut de Chimie, Unite Mixte de Recherche 7177, CNRS, Universite de Strasbourg, 67000 Strasbourg, France"
Journal Title:		Plant Cell
Year:		2019
Volume:		20191018
Issue:		12
Page Number:		2947 - 2972
DOI:		10.1105/tpc.19.00320
ISSN/ISBN:		1532-298X (Electronic) 1040-4651 (Print) 1040-4651 (Linking)
Abstract:		"Flowers are essential but vulnerable plant organs, exposed to pollinators and florivores; however, flower chemical defenses are rarely investigated. We show here that two clustered terpene synthase and cytochrome P450 encoding genes (TPS11 and CYP706A3) on chromosome 5 of Arabidopsis (Arabidopsis thaliana) are tightly coexpressed in floral tissues, upon anthesis and during floral bud development. TPS11 was previously reported to generate a blend of sesquiterpenes. By heterologous coexpression of TPS11 and CYP706A3 in yeast (Saccharomyces cerevisiae) and Nicotiana benthamiana, we demonstrate that CYP706A3 is active on TPS11 products and also further oxidizes its own primary oxidation products. Analysis of headspace and soluble metabolites in cyp706a3 and 35S:CYP706A3 mutants indicate that CYP706A3-mediated metabolism largely suppresses sesquiterpene and most monoterpene emissions from opening flowers, and generates terpene oxides that are retained in floral tissues. In flower buds, the combined expression of TPS11 and CYP706A3 also suppresses volatile emissions and generates soluble sesquiterpene oxides. Florivory assays with the Brassicaceae specialist Plutella xylostella demonstrate that insect larvae avoid feeding on buds expressing CYP706A3 and accumulating terpene oxides. Composition of the floral microbiome appears also to be modulated by CYP706A3 expression. TPS11 and CYP706A3 simultaneously evolved within Brassicaceae and form the most versatile functional gene cluster described in higher plants so far.plantcell;31/12/2947/FX1F1fx1"
Keywords:		Alkyl and Aryl Transferases/genetics/*metabolism Animals Arabidopsis/enzymology/genetics/*metabolism/microbiology Arabidopsis Proteins/chemistry/genetics/*metabolism Cytochrome P-450 Enzyme System/chemistry/genetics/*metabolism Flowers/genetics/*metabolis;
Notes:		"MedlineBoachon, Benoit Burdloff, Yannick Ruan, Ju-Xin Rojo, Rakotoharisoa Junker, Robert R Vincent, Bruno Nicole, Florence Bringel, Francoise Lesot, Agnes Henry, Laura Bassard, Jean-Etienne Mathieu, Sandrine Allouche, Lionel Kaplan, Ian Dudareva, Natalia Vuilleumier, Stephane Miesch, Laurence Andre, Francois Navrot, Nicolas Chen, Xiao-Ya Werck-Reichhart, Daniele eng Research Support, Non-U.S. Gov't England 2019/10/20 Plant Cell. 2019 Dec; 31(12):2947-2972. doi: 10.1105/tpc.19.00320. Epub 2019 Oct 18"