| Title: | Pheromone-induced expression of recombinant proteins in Streptococcus thermophilus |
| Author(s): | Blomqvist T; Steinmoen H; Havarstein LS; |
| Address: | "Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O.Box 5003, 1432, As, Norway" |
| DOI: | 10.1007/s00203-006-0162-0 |
| ISSN/ISBN: | 0302-8933 (Print) 0302-8933 (Linking) |
| Abstract: | "A locus encoding proteins with high homology to the pneumococcal BlpABCHR quorum-sensing system was identified in Streptococcus thermophilus LMG 18311. The BlpABCHR system regulates bacteriocin production in Streptococcus pneumoniae by monitoring the extracellular concentration of a peptide-pheromone encoded by blpC. The homologous system in S. thermophilus, termed StbABCHR, contains a corresponding gene (stbC) encoding a possible peptide-pheromone (STP) that presumably controls bacteriocin production in S. thermophilus. We synthesized this peptide and found that it activates transcription of a gusA reporter gene placed behind the promoter of the bacteriocin-like gene stbD. Furthermore, deletion mapping and mutational analysis of the stbD promoter region were used to identify a degenerated direct repeat motif required for STP induced GusA expression. Our findings provide strong evidence that STP regulates bacteriocin production in S. thermophilus LMG 18311, and show that the StbABCHR quorum-sensing system can be exploited for inducible expression of recombinant proteins in this bacterial species" |
| Keywords: | "Bacteriocins/*biosynthesis/genetics DNA Mutational Analysis Enzyme Induction *Gene Expression Regulation, Bacterial Pheromones/*pharmacology Promoter Regions, Genetic/genetics Quorum Sensing/*physiology Recombinant Proteins/*biosynthesis Streptococcus the;" |
| Notes: | "MedlineBlomqvist, Trinelise Steinmoen, Hilde Havarstein, Leiv Sigve eng Research Support, Non-U.S. Gov't Germany 2006/08/26 Arch Microbiol. 2006 Dec; 186(6):465-73. doi: 10.1007/s00203-006-0162-0. Epub 2006 Aug 24" |
