| Title: | Structure and mode of peptide binding of pheromone receptor PrgZ |
| Author(s): | Berntsson RP; Schuurman-Wolters GK; Dunny G; Slotboom DJ; Poolman B; |
| Address: | "Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, University of Groningen, 9747 AG Groningen, The Netherlands" |
| ISSN/ISBN: | 1083-351X (Electronic) 0021-9258 (Print) 0021-9258 (Linking) |
| Abstract: | "We present the crystal structure of the pheromone receptor protein PrgZ from Enterococcus faecalis in complex with the heptapeptide cCF10 (LVTLVFV), which is used in signaling between conjugative recipient and donor cells. Comparison of PrgZ with homologous oligopeptide-binding proteins (AppA and OppA) explains the high specificity of PrgZ for hydrophobic heptapeptides versus the promiscuity of peptide binding in the homologous proteins" |
| Keywords: | "Amino Acid Sequence Bacterial Proteins/*chemistry Binding Sites Calorimetry, Differential Scanning Carrier Proteins/*chemistry Crystallography, X-Ray *Enterococcus faecalis Hydrogen Bonding Hydrophobic and Hydrophilic Interactions Models, Molecular Oligop;" |
| Notes: | "MedlineBerntsson, Ronnie P-A Schuurman-Wolters, Gea K Dunny, Gary Slotboom, Dirk-Jan Poolman, Bert eng R01 GM049530/GM/NIGMS NIH HHS/ GM49530/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2012/09/06 J Biol Chem. 2012 Oct 26; 287(44):37165-70. doi: 10.1074/jbc.M112.386334. Epub 2012 Sep 4" |
