« Previous AbstractPheromone cCF10 and plasmid pCF10-encoded regulatory molecules act post-transcriptionally to activate expression of downstream conjugation functions    Next AbstractA simple dynamic flow-through exposure system for assessment of biological activity of complex organic mixtures to mammalian cells in vitro »

Mol Microbiol

Title:		Pheromone-inducible expression of an aggregation protein in Enterococcus faecalis requires interaction of a plasmid-encoded RNA with components of the ribosome
Author(s):		Bensing BA; Dunny GM;
Address:		"Department of Microbiology and Institute for Advanced Studies in Biological Process Technology, University of Minnesota, Minneapolis 55455, USA"
Journal Title:		Mol Microbiol
Year:		1997
Volume:		24
Issue:		2
Page Number:		295 - 308
DOI:		10.1046/j.1365-2958.1997.3311709.x
ISSN/ISBN:		0950-382X (Print) 0950-382X (Linking)
Abstract:		"Transfer of the conjugative plasmid pCF10 from Enterococcus faecalis donor strains is induced by a peptide pheromone (cCF10) secreted by recipient cells. High-efficiency transfer requires expression of an aggregation protein (Asc10) encoded by the prgB gene and positively regulated by genes in a region 3-5 kb upstream, containing prgQ-R-S. Transcriptional fusion data reported here support the results of recent molecular analysis of the 5' ends of prgB transcripts which indicated that prgB transcription occurs by readthrough from the prgQ promoter. A 530-nucleotide prgQ-encoded RNA molecule (Q(L)) with rRNA-like domains is required for Asc10 production. Q(L) and cCF10 were found to interact with the L6 and S5 ribosomal proteins, respectively. Mutational analysis of Q(L) indicates that this RNA may also directly interact with 16S RNA. Q(L) is present in ribosomes translating the prgB message, and pheromone cCF10 may affect the association of this RNA with translation complexes. Results suggest that the positive regulatory molecules act post-transcriptionally on the polycistronic message and modify a ribosome population to enhance pheromone-induced translation of prgB"
Keywords:		"Amino Acid Sequence Bacterial Proteins/*genetics/*metabolism Base Sequence Chromosome Mapping Enterococcus faecalis/*genetics/*metabolism *Gene Expression Regulation, Bacterial Membrane Proteins/*genetics/*metabolism Molecular Sequence Data Molecular Stru;"
Notes:		"MedlineBensing, B A Dunny, G M eng 1RO1GM49530/GM/NIGMS NIH HHS/ 1T32AI07421/AI/NIAID NIH HHS/ Research Support, U.S. Gov't, P.H.S. England 1997/04/01 Mol Microbiol. 1997 Apr; 24(2):295-308. doi: 10.1046/j.1365-2958.1997.3311709.x"