| Title: | Identification of Biomarker Volatile Organic Compounds Released by Three Stored-Grain Insect Pests in Wheat |
| Author(s): | Cai L; Macfadyen S; Hua B; Zhang H; Xu W; Ren Y; |
| Address: | "State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Northwest A&F University, Xianyang 712100, China. Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia. College of Science, Health, Engineering and Education, Murdoch University, Murdoch, WA 6150, Australia" |
| DOI: | 10.3390/molecules27061963 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Monitoring and early detection of stored-grain insect infestation is essential to implement timely and effective pest management decisions to protect stored grains. We report a reliable analytical procedure based on headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) to assess stored-grain infestation through the detection of volatile compounds emitted by insects. Four different fibre coatings were assessed; 85 microm CAR/PDMS had optimal efficiency in the extraction of analytes from wheat. The headspace profiles of volatile compounds produced by Tribolium castaneum (Herbst), Rhyzopertha dominica (Fabricius), and Sitophilus granarius (Linnaeus), either alone or with wheat, were compared with those of non-infested wheat grains. Qualitative analysis of chromatograms showed the presence of different volatile compound profiles in wheat with pest infestation compared with the wheat controls. Wheat-specific and insect-specific volatile compounds were identified, including the aggregation pheromones, dominicalure-1 and dominicalure-2, from R. dominica, and benzoquinones homologs from T. castaneum. For the first time, the presence of 3-hydroxy-2-butanone was reported from S. granarius, which might function as an alarm pheromone. These identified candidate biomarker compounds can be utilized in insect surveillance and monitoring in stored grain to safeguard our grain products in future" |
| Keywords: | Animals Biomarkers Edible Grain Insecta *Triticum *Volatile Organic Compounds Rhyzopertha dominica Spme-gc-ms Sitophilus granarius Tribolium castaneum headspace solid-phase microextraction (HS-SPME) stored-product insect pests volatile organic compounds (; |
| Notes: | "MedlineCai, Lijun Macfadyen, Sarina Hua, Baozhen Zhang, Haochuan Xu, Wei Ren, Yonglin eng DE160100382/Australian Research Council/ 132120030/Chinese Civil Services Bureau of Fujian Province for Early Career Researcher/ Switzerland 2022/03/27 Molecules. 2022 Mar 17; 27(6):1963. doi: 10.3390/molecules27061963" |
