| Title: | A volatolomic approach using cerumen as biofluid to diagnose bovine intoxication by Stryphnodendron rotundifolium |
| Author(s): | Barbosa JMG; Fernandes Rodrigues MK; David LC; TC ES; Fortuna Lima DA; Pereira NZ; D'Alessandro EB; de Oliveira AE; Jorge da Cunha PH; Fioravanti MCS; Antoniosi Filho NR; |
| Address: | "Laboratory of Extraction and Separation Methods (LAMES), Institute of Chemistry, Federal University of Goias (UFG), Goiania, GO, Brazil. Veterinary and Zootechnical School (EVZ), Federal University of Goias (UFG), Goiania, GO, Brazil. Laboratory of Theoretical and Computational Chemistry (LQTC), Institute of Chemistry, Federal University of Goias (UFG), Goiania, GO, Brazil" |
| ISSN/ISBN: | 1099-0801 (Electronic) 0269-3879 (Linking) |
| Abstract: | "An innovative volatolomic approach employs the detection of biomarkers present in cerumen (earwax) to identify cattle intoxication by Stryphnodendron rotundifolium Mart., Fabaceae (popularly known as barbatimao). S. rotundifolium is a poisonous plant with the toxic compound undefined and widely distributed throughout the Brazilian territory. Cerumen samples from cattle of two local Brazilian breeds ('Curraleiro Pe-Duro' and 'Pantaneiro') were collected during an experimental intoxication protocol and analyzed using headspace (HS)/GC-MS followed by multivariate analysis (genetic algorithm for a partial least squares, cluster analysis, and classification and regression trees). A total of 106 volatile organic metabolites were identified in the cerumen samples of bovines. The intoxication by S. rotundifolium influenced the cerumen volatolomic profile of the bovines throughout the intoxication protocol. In this way, it was possible to detect biomarkers for cattle intoxication. Among the biomarkers, 2-octyldecanol and 9-tetradecen-1-ol were able to discriminate all samples between intoxicated and nonintoxicated bovines. The cattle intoxication diagnosis by S. rotundifolium was accomplished by applying the cerumen analysis using HS/GC-MS, in an easy, accurate, and noninvasive way. Thus, the proposed bioanalytical chromatography protocol is a useful tool in veterinary applications to determine this kind of intoxication" |
| Keywords: | Animals Biomarkers/analysis Brazil Cattle Cattle Diseases/*diagnosis Cerumen/*chemistry Fabaceae/*adverse effects Gas Chromatography-Mass Spectrometry Least-Squares Analysis Male Plant Poisoning/*diagnosis/*veterinary Volatile Organic Compounds/analysis H; |
| Notes: | "MedlineBarbosa, Joao Marcos G Fernandes Rodrigues, Milenna K David, Lurian C E Silva, Taynara C Fortuna Lima, Danielly A Pereira, Naiara Z D'Alessandro, Emmanuel B de Oliveira, Anselmo E Jorge da Cunha, Paulo H Fioravanti, Maria Clorinda S Antoniosi Filho, Nelson R eng 407774/2013-0/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ 312280/2016-5/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ 88882.386430/2019-01 - Finance Code: 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ Fundacao de Apoio a Pesquisa, Universidade Federal de Goias/ England 2020/07/01 Biomed Chromatogr. 2020 Nov; 34(11):e4935. doi: 10.1002/bmc.4935. Epub 2020 Jul 17" |
