| Title: | "Quali-Quantitative Characterization of Volatile and Non-Volatile Compounds in Protium heptaphyllum (Aubl.) Marchand Resin by GC-MS Validated Method, GC-FID and HPLC-HRMS(2)" |
| Author(s): | Asteggiano A; Occhipinti A; Capuzzo A; Mecarelli E; Aigotti R; Medana C; |
| Address: | "Department of Molecular Biotechnology and Health Sciences, University of Torino, Via Pietro Giuria 5, 10125 Torino, Italy. Biosfered S.r.l. Via Paolo Veronese 202, 10148 Turin, Italy. Abel Nutraceuticals S.r.l. Via Paolo Veronese 202, 10148 Turin, Italy" |
| DOI: | 10.3390/molecules26051447 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Protium heptaphyllum (Aubl.) Marchand (PH) trees are endemic to the tropical region of South America, mostly Brazil. Antibacterial, antinociceptive, anti-inflammatory, anxiolytic, antidepressant and anti-hyperlipidemic/anti-hypercholesterolemic effects were reported for its resinous exudate Protiumheptaphyllum resin (PHR). This work aims to provide a qualitative and quantitative consistent chemical profiling of the major constituents of this resin and two extracts enriched in acid (acidic triterpene concentrated extract, ATCE) and neutral triterpenes (alpha and beta-amyrin concentrated extract, AMCE). GC-MS/GC-FID was used for volatile terpene fraction, a validated GC-MS method was developed for quantification of neutral alpha and beta-amyrin and HPLC-APCI HRMS(2) was used for acidic triterpenes analysis. The chemical investigation reported 29 molecules, including 14 volatile terpenes, 6 neutral triterpenes and 11 acid triterpenes. The most abundant compounds were alpha-amyrin (251.28 g kg(-1), 123.98 g kg(-1) and 556.82 g kg(-1) in PHR, ATCE and AMCE, respectively), beta-amyrin (172.66 g kg(-1), 95.39 g kg(-1) and 385.58 g kg(-1) in PHR, ATCE and AMCE, respectively), 3-oxo-tirucalla-7,24-dien-21-oic acid (80.64 g kg(-1), 157.10 g kg(-1) and 15.31 g kg(-1) in PHR, ATCE and AMCE, respectively) and 3alpha-hydroxy-tirucalla-8,24-dien-21-oic acid (77.71 g kg(-1), 130.40 g kg(-1) and 11.64 g kg(-1) in PHR, ATCE and AMCE, respectively). Results showed specific enrichment of acidic and neutral triterpenoids in the two respective extracts" |
| Keywords: | "Burseraceae/*chemistry Chromatography, High Pressure Liquid/methods Gas Chromatography-Mass Spectrometry/methods Pentacyclic Triterpenes/analysis Plant Extracts/analysis/chemistry Reproducibility of Results Resins, Plant/*chemistry Terpenes/analysis Trite;" |
| Notes: | "MedlineAsteggiano, Alberto Occhipinti, Andrea Capuzzo, Andrea Mecarelli, Enrica Aigotti, Riccardo Medana, Claudio eng Switzerland 2021/04/04 Molecules. 2021 Mar 7; 26(5):1447. doi: 10.3390/molecules26051447" |
