| Title: | "Pheromone-gland-specific fatty-acyl reductase in the adzuki bean borer, Ostrinia scapulalis (Lepidoptera: Crambidae)" |
| Author(s): | Antony B; Fujii T; Moto K; Matsumoto S; Fukuzawa M; Nakano R; Tatsuki S; Ishikawa Y; |
| Address: | "Laboratory of Applied Entomology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan" |
| DOI: | 10.1016/j.ibmb.2008.10.008 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "The adzuki bean borer moth, Ostrinia scapulalis, uses a mixture of (E)-11- and (Z)-11-tetradecenyl acetates as a sex pheromone. At a step in the pheromone biosynthetic pathway, fatty-acyl precursors are converted to corresponding alcohols by an enzyme, fatty-acyl reductase (FAR). Here we report the cloning of FAR-like genes expressed in the pheromone gland of female O. scapulalis, and the characterization of a single pheromone-gland-specific FAR (pgFAR) and its functional assay using an insect cell expression system. As many as thirteen FAR-like genes (FAR-I-FAR-XIII) were expressed in the pheromone gland of O. scapulalis; however, only one (FAR-XIII) was pheromone-gland-specific. The deduced amino acid sequence of FAR-XIII predicted a 462-aa protein with a conserved NAD(P)H-binding motif in the N-terminal region, showing overall identity of 34% with the pgFAR of Bombyx mori. A functional assay using Sf9 cells transfected with an expression vector containing the open reading frame of the FAR-XIII gene has proven that FAR-XIII protein has the ability to convert a natural substrate, (Z)-11-tetradecenoic acid, to a corresponding alcohol, (Z)-11-tetradecenol" |
| Keywords: | "Amino Acid Motifs Amino Acid Sequence Animals Cloning, Molecular Exocrine Glands/chemistry/enzymology Female Insect Proteins/chemistry/genetics/*metabolism Molecular Sequence Data Moths/chemistry/*enzymology/genetics Organ Specificity Oxidoreductases/chem;" |
| Notes: | "MedlineAntony, Binu Fujii, Takeshi Moto, Ken'ichi Matsumoto, Shogo Fukuzawa, Mai Nakano, Ryo Tatsuki, Sadahiro Ishikawa, Yukio eng Research Support, Non-U.S. Gov't England 2008/12/02 Insect Biochem Mol Biol. 2009 Feb; 39(2):90-5. doi: 10.1016/j.ibmb.2008.10.008. Epub 2008 Oct 26" |
