| Title: | Pyrokinin/PBAN radio-receptor assay: development and application for the characterization of a putative receptor from the pheromone gland of Heliothis peltigera |
| Author(s): | Altstein M; Ben-Aziz O; Daniel S; Zeltser I; Gilon C; |
| Address: | "Department of Entomology, The Volcani Center, 50250, Bet Dagan, Israel. vinnie2@netvision.net.il" |
| DOI: | 10.1016/s0196-9781(01)00479-x |
| ISSN/ISBN: | 0196-9781 (Print) 0196-9781 (Linking) |
| Abstract: | "A radio-receptor assay (RRA) for the insect pyrokinin/PBAN family has been developed. The development involved examination of the ligand (3H-tyrosyl-PBAN28-33NH2)-receptor interaction under various incubation conditions and variations on sex pheromone gland membrane preparation. Application of the RRA for a partial characterization of the putative pyrokinin/PBAN receptor in the pheromone gland of H. peltigera revealed age-dependence of its expression. Pharmacological characterization revealed a high correlation between the binding-affinity to the receptor of various PBAN-derived peptides and their in vivo pheromonotropic bioactivity, and shed light on the interaction of backbone cyclic and linear ([Arg27,D-Phe30]PBAN28-33NH2) PBAN antagonists with the receptor" |
| Keywords: | "Animals Binding Sites Binding, Competitive Buffers Cell Membrane/drug effects/metabolism Chemoreceptor Cells/*metabolism Dose-Response Relationship, Drug Female Hydrogen-Ion Concentration Insect Hormones/chemistry/metabolism Ligands Moths Neuropeptides/*a;" |
| Notes: | "MedlineAltstein, M Ben-Aziz, O Daniel, S Zeltser, I Gilon, C eng Research Support, Non-U.S. Gov't 2001/08/22 Peptides. 2001 Sep; 22(9):1379-89. doi: 10.1016/s0196-9781(01)00479-x" |
