« Previous AbstractOptical waveguide sensor of volatile organic compounds based on PTA thin film    Next AbstractStructure and function of a peptide pheromone family that stimulate the vomeronasal sensory system in mice »

Plant Cell Physiol

Title:		Stable nuclear transformation of the Closterium peracerosum-strigosum-littorale complex
Author(s):		Abe J; Hori S; Tsuchikane Y; Kitao N; Kato M; Sekimoto H;
Address:		"Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo, 112-8681 Japan. junabe@fc.jwu.ac.jp"
Journal Title:		Plant Cell Physiol
Year:		2011
Volume:		20110729
Issue:		9
Page Number:		1676 - 1685
DOI:		10.1093/pcp/pcr103
ISSN/ISBN:		1471-9053 (Electronic) 0032-0781 (Linking)
Abstract:		"Although charophycean algae form a relevant monophyly with embryophytes and hence occupy a fundamental place in the development of Streptophyta, no tools for genetic transformation in these organisms have been developed. Here we present the first stable nuclear transformation system for the unicellular Zygnematales, the Closterium peracerosum-strigosum-littorale complex (C. psl complex), which is one of the most useful organisms for experimental research on charophycean algae. When a vector, pSA106, containing the dominant selectable marker ble (phleomycin-resistant) gene and a reporter cgfp (Chlamydomonas-adapted green fluorescent protein) gene was introduced into cells via particle bombardment, a total of 19 phleomycin-resistant cells were obtained in the presence of a low concentration of phleomycin. Six isogenic strains isolated using conditioned medium showed consecutive cgfp expression and long-term stability for phleomycin resistance. DNA analyses verified single or tandem/redundant integration of ~10 copies of pSA106 into the C. psl complex genome. We also constructed an overexpression vector, pSA1102, and then integrated a CpPI gene encoding minus-specific sex pheromone into pSA1102. Ectopic overexpression of CpPI and the pheromonal function were confirmed when the vector pSA1102_CpPI was introduced into mt(+) cells. The present efficient transformation system for the C. psl complex should provide not only a basis for molecular investigation of Closterium but also an insight into important processes in early development and evolution of Streptophyta"
Keywords:		"Closterium/*genetics DNA, Plant/genetics Gene Expression Regulation, Plant *Gene Transfer Techniques Genes, Reporter Genetic Vectors Plasmids/genetics *Transformation, Genetic;"
Notes:		"MedlineAbe, Jun Hori, Sachie Tsuchikane, Yuki Kitao, Naoko Kato, Misako Sekimoto, Hiroyuki eng Research Support, Non-U.S. Gov't Japan 2011/08/02 Plant Cell Physiol. 2011 Sep; 52(9):1676-85. doi: 10.1093/pcp/pcr103. Epub 2011 Jul 29"