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Front Microbiol

Title:		Dissemination of Linezolid Resistance Through Sex Pheromone Plasmid Transfer in Enterococcus faecalis
Author(s):		Zou J; Tang Z; Yan J; Liu H; Chen Y; Zhang D; Zhao J; Tang Y; Zhang J; Xia Y;
Address:		"Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Department of Urologic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Department of Laboratory Medicine, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, China"
Journal Title:		Front Microbiol
Year:		2020
Volume:		20200604
Issue:
Page Number:		1185 -
DOI:		10.3389/fmicb.2020.01185
ISSN/ISBN:		1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking)
Abstract:		"Despite recent recognition of the ATP-binding cassette protein OptrA as an important mediator of linezolid resistance in Enterococcus faecalis worldwide, the mechanisms of optrA gene acquisition and transfer remain poorly understood. In this study, we performed comprehensive molecular and phenotypic profiling of 44 optrA-carrying E. faecalis clinical isolates with linezolid resistance. Pulse-field gel electrophoresis and DNA hybridization revealed the presence of optrA in the plasmid in 26 (59%) isolates and in the chromosome in 18 (41%) isolates. Conjugation experiments showed a successful transfer of optrA in 88.5% (23/26) of isolates carrying optrA in plasmids while no transfer occurred in any isolates carrying optrA in the chromosome (0/18). All 23 transconjugants exhibited in vitro resistance to linezolid and several other antibiotics and were confirmed to contain optrA and other resistance genes. Plasmid typing demonstrated a predominance (18/23,78%) of rep (9)-type plasmids (pCF10 prototype) known to be the best studied sex pheromone responsive plasmids. Full plasmid genome sequencing of one isolate revealed the presence of drug resistance genes (optrA and fexA) and multiple sex pheromone response genes in the same plasmid, which represents the first sex pheromone responsive plasmid carrying optrA from a clinical isolate. PCR-based genotyping revealed the presence of three key sex pheromone response genes (prgA, prgB, and prgC) in 23 optrA-carrying isolates. Finally, functional studies of these isolates by clumping induction assay detected different degrees of clumping in 17 isolates. Our analysis suggests that optrA-mediated linezolid resistance can be widely disseminated through sex pheromone plasmid transfer"
Keywords:		Enterococcus faecalis linezolid optrA sex pheromone plasmid transmission;
Notes:		"PubMed-not-MEDLINEZou, Jiaqi Tang, Zhaobing Yan, Jia Liu, Hang Chen, Yingzhu Zhang, Dawei Zhao, Jinxin Tang, Yu Zhang, Jing Xia, Yun eng Switzerland 2020/06/26 Front Microbiol. 2020 Jun 4; 11:1185. doi: 10.3389/fmicb.2020.01185. eCollection 2020"