| Title: | Identification of volatile organic compounds secreted from cancer tissues and bacterial cultures |
| Author(s): | Buszewski B; Ulanowska A; Ligor T; Jackowski M; Klodzinska E; Szeliga J; |
| Address: | "Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland. bbusz@chem.uni.torun.pl" |
| Journal Title: | J Chromatogr B Analyt Technol Biomed Life Sci |
| DOI: | 10.1016/j.jchromb.2008.04.038 |
| ISSN/ISBN: | 1570-0232 (Print) 1570-0232 (Linking) |
| Abstract: | "The early cancer diagnosis increases the possibility of total recovery. The infection of Helicobacter pylori is associated with gastric cancer, the second most common cancer in the world. The determination of volatile organic compounds (VOCs) excreted by stomach tissue and bacteria culture has been investigated. Solid-phase microextraction (SPME) was used for preconcentration and the determination was accomplished by gas chromatography coupled with mass spectrometry (GC/MS). The samples of tissue were taken from five patients (ten samples) with stomach cancer and normal (non-cancerous) segments from other parts of the stomach were used as a control. Eighteen compounds were identified in stomach tissue and seven of them were present both in healthy and cancer tissue. These compounds assumed to be endogenous and acetone ratio (AR) was calculated for ethanol, butane, carbon disulfide, 1-propanol, 2-butanone and 2-pentanone. The data shows that amount of 1-propanol and carbon disulfide in the gaseous composition is higher in cancer tissue than in normal tissue. Eight compounds were identified both in bacteria and tissue. These data suggest that bacteria present in the stomach might cause the increase in the concentration of 1-propanol and carbon disulfide in emission from cancer tissue" |
| Keywords: | Bacteria/*metabolism Case-Control Studies Female Gas Chromatography-Mass Spectrometry Humans Male Organic Chemicals/*chemistry Sensitivity and Specificity Stomach Neoplasms/*metabolism Volatilization; |
| Notes: | "MedlineBuszewski, Boguslaw Ulanowska, Agnieszka Ligor, Tomasz Jackowski, Marek Klodzinska, Ewa Szeliga, Jacek eng Research Support, Non-U.S. Gov't Netherlands 2008/05/21 J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jun 1; 868(1-2):88-94. doi: 10.1016/j.jchromb.2008.04.038. Epub 2008 May 3" |
