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Microb Cell Fact

Title:		Bioprospection of actinobacteria derived from freshwater sediments for their potential to produce antimicrobial compounds
Author(s):		Zothanpuia; Passari AK; Leo VV; Chandra P; Kumar B; Nayak C; Hashem A; Abd Allah EF; Alqarawi AA; Singh BP;
Address:		"Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram, 796004, India. SAIF, CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow, 226012, India. University of Mysore, Manasagangotri, Mysore, India. Botany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh, 11451, Saudi Arabia. Mycology and Plant Disease Survey Department, Plant Pathology Research Institute, ARC, Giza, 12511, Egypt. Department of Plant Production, Faculty of Food & Agricultural Sciences, P.O. Box. 2460, Riyadh, 11451, Saudi Arabia. Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram, 796004, India. bhimpratap@gmail.com"
Journal Title:		Microb Cell Fact
Year:		2018
Volume:		20180505
Issue:		1
Page Number:		68 -
DOI:		10.1186/s12934-018-0912-0
ISSN/ISBN:		1475-2859 (Electronic) 1475-2859 (Linking)
Abstract:		"BACKGROUND: Actinobacteria from freshwater habitats have been explored less than from other habitats in the search for compounds of pharmaceutical value. This study highlighted the abundance of actinobacteria from freshwater sediments of two rivers and one lake, and the isolates were studied for their ability to produce antimicrobial bioactive compounds. RESULTS: 16S rRNA gene sequencing led to the identification of 84 actinobacterial isolates separated into a common genus (Streptomyces) and eight rare genera (Nocardiopsis, Saccharopolyspora, Rhodococcus, Prauserella, Amycolatopsis, Promicromonospora, Kocuria and Micrococcus). All strains that showed significant inhibition potentials were found against Gram-positive, Gram-negative and yeast pathogens. Further, three biosynthetic genes, polyketide synthases type II (PKS II), nonribosomal peptide synthetases (NRPS) and aminodeoxyisochorismate synthase (phzE), were detected in 38, 71 and 29% of the strains, respectively. Six isolates based on their antimicrobial potentials were selected for the detection and quantification of standard antibiotics using ultra performance liquid chromatography (UPLC-ESI-MS/MS) and volatile organic compounds (VOCs) using gas chromatography mass spectrometry (GC/MS). Four antibiotics (fluconazole, trimethoprim, ketoconazole and rifampicin) and 35 VOCs were quantified and determined from the methanolic crude extract of six selected Streptomyces strains. CONCLUSION: Infectious diseases still remain one of the leading causes of death globally and bacterial infections caused millions of deaths annually. Culturable actinobacteria associated with freshwater lake and river sediments has the prospects for the production of bioactive secondary metabolites"
Keywords:		Actinobacteria/*pathogenicity Anti-Infective Agents/metabolism Fresh Water/*chemistry Actinobacteria Gc-ms Nrps Pksii Uplc-esi-ms/ms VOCs phzE;
Notes:		"MedlineZothanpuia Passari, Ajit Kumar Leo, Vincent Vineeth Chandra, Preeti Kumar, Brijesh Nayak, Chandra Hashem, Abeer Abd Allah, Elsayed Fathi Alqarawi, Abdulaziz A Singh, Bhim Pratap eng 102/IFD/SAN/4290-4291/2016-2017/Department of Biotechnology , Ministry of Science and Technology/ England 2018/05/08 Microb Cell Fact. 2018 May 5; 17(1):68. doi: 10.1186/s12934-018-0912-0"