| Title: | A multiplatform metabolomic approach to characterize fecal signatures of negative postnatal events in chicks: a pilot study |
| Author(s): | Beauclercq S; Lefevre A; Montigny F; Collin A; Tesseraud S; Leterrier C; Emond P; Guilloteau LA; |
| Address: | "1BOA, INRA, Universite de Tours, 37380 Nouzilly, France. GRID: grid.418065.e 2Universite de Tours, PST Analyse des systemes biologiques, Tours, France. ISNI: 0000 0001 2182 6141. GRID: grid.12366.30 PRC, INRA, CNRS, Universite de Tours, IFCE, 37380 Nouzilly, France. 4UMR 1253, iBrain, Universite de Tours, Inserm, Tours, France. ISNI: 0000 0001 2182 6141. GRID: grid.12366.30 5CHRU de Tours, Service de Medecine Nucleaire In Vitro, Tours, France. ISNI: 0000 0004 1765 1600. GRID: grid.411167.4" |
| DOI: | 10.1186/s40104-019-0335-8 |
| ISSN/ISBN: | 1674-9782 (Print) 2049-1891 (Electronic) 1674-9782 (Linking) |
| Abstract: | "BACKGROUND: Negative experiences in early life can induce long-lasting effects on the welfare, health, and performance of farm animals. A delayed placement of chicks in rearing houses has negative effects on their performance, and results in fecal-specific odors detectable by rats. Based on this observation, the volatile organic compounds (VOCs) and metabolites from the feces of 12-day-old chickens were screened for early markers of response to negative events using gas-chromatography and liquid-chromatography coupled with mass spectrometry (GC-MS, LC-HRMS). RESULTS: The low reproducibility of solid-phase micro-extraction of the VOCs followed by GC-MS was not suitable for marker discovery, in contrast to liquid extraction of metabolites from freeze-dried feces followed by GC-MS or LC-HRMS analysis. Therefore, the fecal metabolome from 12-day-old chicks having experienced a normal or delayed placement were recorded by GC-MS and LC-HRMS in two genotypes from two experiments. From both experiments, 25 and 35 metabolites, respectively explaining 81% and 45% of the difference between delayed and control chickens, were identified by orthogonal partial least-squares discriminant analysis from LC-HRMS and GC-MS profiling. CONCLUSION: The sets of molecules identified will be useful to better understand the chicks' response to negative events over time and will contribute to define stress or welfare biomarkers." |
| Keywords: | Biomarker Chick Feces Gc-ms Lc-hrms Negative postnatal event; |
| Notes: | "PubMed-not-MEDLINEBeauclercq, Stephane Lefevre, Antoine Montigny, Frederic Collin, Anne Tesseraud, Sophie Leterrier, Christine Emond, Patrick Guilloteau, Laurence A eng England 2019/04/23 J Anim Sci Biotechnol. 2019 Apr 9; 10:21. doi: 10.1186/s40104-019-0335-8. eCollection 2019" |
