| Title: | Dynamic microbial succession of Shanxi aged vinegar and its correlation with flavor metabolites during different stages of acetic acid fermentation |
| Author(s): | Zhu Y; Zhang F; Zhang C; Yang L; Fan G; Xu Y; Sun B; Li X; |
| Address: | "Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, 100048, China. Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), Beijing, 100048, China. School of Food and Chemical Engineering, Beijing Technology and Business University, No.33, Fucheng Road, Beijing, 100048, China. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, 100048, China. lixt@th.btbu.edu.cn. School of Food and Chemical Engineering, Beijing Technology and Business University, No.33, Fucheng Road, Beijing, 100048, China. lixt@th.btbu.edu.cn" |
| DOI: | 10.1038/s41598-018-26787-6 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Shanxi aged vinegar (SAV), one of the famous Chinese vinegars, is produced by multispecies solid-state fermentation in which the acetic acid fermentation stage (AAF) is especially important. However, how bacterial succession and their metabolites change along with the different stages of AAF is still poorly understood. In this study, we investigated the dynamic bacterial succession and flavor formation in three batches of SAV using high-throughput sequencing and metabolomics approaches. It is interesting to find that AAF can be divided into three stages based on its bacterial community succession (early stage, days 0-4; medium stage, days 5-21; and later stage, days 22-26). Pantoea, Pediococcus, Lactococcus and Rhizobium played an important role in the early stage; Lactobacillus was dominant in the medium stage (67.72%); and Acetobacter, Komagataeibacter and Kroppenstedtia were the key bacteria in the later stage. A total of seven organic acids and 42 volatile constituents (esters, alcohol, ketones and aldehydes) were detected during the AAF. Spearman correlation analysis showed a significant correlation between the bacterial community and these flavor metabolites during the AAF of the SAV. This is the first report to explore the relationships between volatile flavor metabolites and bacterial community succession by a three-staged method and provide theoretical support for a flavor formation mechanism in traditional SAV" |
| Keywords: | Acetic Acid/*metabolism Bacteria/*classification/*metabolism *Biota Fermentation *Food Microbiology High-Throughput Nucleotide Sequencing Metabolomics Metagenomics Volatile Organic Compounds/metabolism; |
| Notes: | "MedlineZhu, Yunping Zhang, Feifei Zhang, Chengnan Yang, Li Fan, Guangsen Xu, Youqiang Sun, Baoguo Li, Xiuting eng Research Support, Non-U.S. Gov't England 2018/06/07 Sci Rep. 2018 Jun 5; 8(1):8612. doi: 10.1038/s41598-018-26787-6" |
