| Title: | Odorant-binding Protein 10 From Bradysia odoriphaga (Diptera: Sciaridae) Binds Volatile Host Plant Compounds |
| Author(s): | Zhu J; Wang F; Zhang Y; Yang Y; Hua D; |
| Address: | "Hubei Engineering Technology Center for Pest Forewarning and Management, Institute of Insect Sciences, College of Agriculture, Yangtze University, Jingzhou 434000, Hubei, China. Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. Institute of Agricultural Quality Standards and Testing Technology Research, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products, Wuhan 430064, Hubei, China" |
| ISSN/ISBN: | 1536-2442 (Electronic) 1536-2442 (Linking) |
| Abstract: | "Bradysia odoriphaga (Diptera: Sciaridae) is a major insect pest of seven plant families including 30 commercial crops in Asia. The long-term use of chemical pesticides leads to problems such as insect resistance, environmental issues, and food contamination. Against this background, a novel pest control method should be developed. In insects, odorant-binding proteins (OBPs) transport odor molecules, including pheromones and plant volatiles, to olfactory receptors. Here, we expressed and characterized the recombinant B. odoriphaga OBP BodoOBP10, observing that it could bind the sulfur-containing compounds diallyl disulfide and methyl allyl disulfide with Ki values of 8.01 muM and 7.00 muM, respectively. Homology modeling showed that the BodoOBP10 3D structure was similar to that of a typical OBP. Both diallyl disulfide and methyl allyl disulfide bound to the same site on BodoOBP10, mediated by interactions with six hydrophobic residues Met70, Ile75, Thr89, Met90, Leu93, and Leu94, and one aromatic residue, Phe143. Furthermore, silencing BodoOBP10 expression via RNAi significantly reduced the electroantennogram (EAG) response to diallyl disulfide and methyl allyl disulfide. These findings suggest that BodoOBP10 should be involved in the recognition and localization of host plants" |
| Keywords: | "Animals *Diptera/genetics/metabolism *Receptors, Odorant/metabolism Nematocera Insect Proteins/metabolism Bradysia odoriphaga RNAi electroantennogram ligand binding assays odorant-binding protein 10;" |
| Notes: | "MedlineZhu, Jiaqi Wang, Fu Zhang, Youjun Yang, Yuting Hua, Dengke eng Zhang Youjun's Laboratory/ Institute of Vegetables and Flowers/ Chinese Academy of Agricultural Sciences/ 32102202/National Natural Science Foundation of China/ CARS-24-C-02/China Agriculture Research System/ 2023/02/03 J Insect Sci. 2023 Jan 1; 23(1):7. doi: 10.1093/jisesa/iead004" |
