| Title: | Effect of hydroxy safflower yellow A on myocardial apoptosis after acute myocardial infarction in rats |
| Author(s): | Zhou MX; Fu JH; Zhang Q; Wang JQ; |
| Address: | "College of Animal Science and Technology, Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing, China. College of Animal Science and Technology, Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing, China mingxue78@163.com" |
| ISSN/ISBN: | 1676-5680 (Electronic) 1676-5680 (Linking) |
| Abstract: | "This study aimed to investigate the effect of hydroxy safflower yellow A (HSYA) on myocardial apoptosis after acute myocardial infarction (AMI) in rats. We randomly divided 170 male Wistar rats into 6 groups (N = 23): normal control, sham, control, SY (90 mg/kg), HSYA high-dose (HSYA-H, 40 mg/kg), and HSYA low-dose groups (HSYA-L, 20 mg/kg). Myocardial ischemic injury was induced by ligating the anterior descending coronary artery, and the degree of myocardial ischemia was evaluated using electrocardiography and nitroblue tetrazolium staining. Bax and Bcl-2 expressions in the ischemic myocardium were determined using immunohistochemical analysis. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) expression in the myocardium of rats with AMI was determined using reverse transcription-polymerase chain reaction. Compared to rats in the control group, those in the HYSA-H, HSYA-L, and SY groups showed a decrease in the elevated ST segments and an increase in the infarct size. The rats in the drug-treated groups showed a significantly lower percentage of Bax-positive cells and a significantly higher percentage of Bcl-2-positive cells than those in the control group (P < 0.05). Moreover, mRNA expression of PPAR-gamma in the ischemic myocardium of rats in the SY, HSYA-L, and HSYA-H groups was significantly lower than that in the control group (P < 0.05). Thus, HSYA and SY can attenuate myocardial ischemia in rats, possibly by increasing the level of Bcl-2/Bax, and PPAR-gamma may be not a necessary link in this process" |
| Keywords: | Animals Apoptosis/*drug effects Chalcone/*analogs & derivatives/pharmacology Electrocardiography/drug effects Gene Expression/drug effects Heart/drug effects/physiopathology Immunohistochemistry Male Myocardial Infarction/genetics/*metabolism/prevention &; |
| Notes: | "MedlineZhou, M X Fu, J H Zhang, Q Wang, J Q eng Research Support, Non-U.S. Gov't Brazil 2015/05/13 Genet Mol Res. 2015 Apr 10; 14(2):3133-41. doi: 10.4238/2015.April.10.24" |
