| Title: | The 9-lipoxygenase Osr9-LOX1 interacts with the 13-lipoxygenase-mediated pathway to regulate resistance to chewing and piercing-sucking herbivores in rice |
| Author(s): | Zhou G; Ren N; Qi J; Lu J; Xiang C; Ju H; Cheng J; Lou Y; |
| Address: | "State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China; Key Laboratory for Quality Improvement of Agriculture Products of Zhejiang Province, Department of Plant Protection, Zhejiang Agriculture & Forestry University, Lin'an, 311300, China" |
| ISSN/ISBN: | 1399-3054 (Electronic) 0031-9317 (Linking) |
| Abstract: | "Oxylipins produced by the 13-lipoxygenase (LOX) have been reported to play an important role in plant defense responses to herbivores. Yet, the role of oxylipins produced by the 9-LOX pathway in this process remains largely unknown. Here we cloned a gene encoding a chloroplast-localized 9-LOX, Osr9-LOX1, from rice. Transcriptional analysis revealed that herbivore infestation, mechanical wounding and jasmonic acid (JA) treatment either repressed or did not enhance the level of Osr9-LOX1 transcripts at early stages but did at later stages, whereas salicylic acid (SA) treatment quickly increased the transcript level of Osr9-LOX1. Antisense expression of Osr9-lox1 (as-r9lox1) decreased the amount of wound-induced (Z)-3-hexenal but increased levels of striped stem borer (SSB)-induced linolenic acid, JA, SA and trypsin protease inhibitors. These changes were associated with increased resistance in rice to the larvae of the SSB Chilo suppressalis. In contrast, although no significant differences were observed in the duration of the nymph stage or the number of eggs laid by female adults between the brown planthopper (BPH) Nilaparvata lugens that fed on as-r9lox1 lines and BPH that fed on wild-type (WT) rice plants, the survival rate of BPH nymphs that fed on as-r9lox1 lines was higher than that of nymphs that fed on WT plants, possibly because of a higher JA level. The results demonstrate that Osr9-LOX1 plays an important role in regulating an herbivore-induced JA burst and cross-talk between JA and SA, and in controlling resistance in rice to chewing and phloem-feeding herbivores" |
| Keywords: | "Animals Chloroplasts/metabolism Cyclopentanes/analysis *Disease Resistance Female *Gene Expression Regulation, Plant Hemiptera/physiology Herbivory Lepidoptera/physiology Lipoxygenase/*genetics/metabolism Oryza/cytology/*enzymology/genetics/immunology Oxy;" |
| Notes: | "MedlineZhou, Guoxin Ren, Nan Qi, Jingfeng Lu, Jing Xiang, Caiyu Ju, Hongping Cheng, Jiaan Lou, Yonggen eng Research Support, Non-U.S. Gov't Denmark 2014/01/15 Physiol Plant. 2014 Sep; 152(1):59-69. doi: 10.1111/ppl.12148. Epub 2014 Feb 10" |
