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Plant Sci

Title:		A fast and selective gas liquid microextraction of semiochemicals for quantitative analysis in plants
Author(s):		Zhao J; Jin X; Wang X; Yang C; Piao X; Kaw HY; Li D;
Address:		"Department of Chemistry, Key Laboratory of Biological Resources of Changbai Mountain & Functional Molecules (Yanbian University), Ministry of Education, Park Road 977, Yanji City, Jilin Province, 133002, China. Department of Chemistry, Changchun Normal University, Changji North Road 677, Changchun City, Jilin Province, 130032, China. Department of Chemistry, Key Laboratory of Biological Resources of Changbai Mountain & Functional Molecules (Yanbian University), Ministry of Education, Park Road 977, Yanji City, Jilin Province, 133002, China. Electronic address: dhli@ybu.edu.cn"
Journal Title:		Plant Sci
Year:		2020
Volume:		20200623
Issue:
Page Number:		110576 -
DOI:		10.1016/j.plantsci.2020.110576
ISSN/ISBN:		1873-2259 (Electronic) 0168-9452 (Linking)
Abstract:		"A trapping-based gas liquid microextraction (GLME) method coupled with gas chromatography-mass spectrometry (GC-MS) was utilized to qualitatively and quantitatively characterize semiochemicals in plants. The main GLME extraction efficiency associated parameters (heating temperature and extraction time) were optimized. The results obtained from GLME process were compared with those of steam distillation and ultrasonic extraction, and the recovery, peak number and reproducibility were evaluated by using Thuja koraiensis Nakai as a representative plant. Furthermore, the quantitative performances of the GLME in terms of sample amount, recoveries of spiked standards and correlation were systematically evaluated using standard addition method, which gave a good quantitative ability for all the compounds with squares of correlation coefficient (r(2)) of higher than 0.99. Finally, the contents of alpha-pinene, camphene, linalool, alpha-terpinenol, beta-caryophyllene, alpha-caryophyllene, and totarol in Thuja koraiensis Nakai samples were quantified, and their concentrations (SD, n = 3) were; 0.65 (0.06), 0.62 (0.05), 4.12 (0.15), 0.99 (0.08), 1.11 (0.07), 0.63 (0.04), and 21.91 (0.25) mug g(-1), respectively. It was demonstrated that GLME is a powerful sample preparation technique for quantitative and qualitative analysis of plant semiochemicals"
Keywords:		Gas Chromatography-Mass Spectrometry/instrumentation/*methods Liquid Phase Microextraction/instrumentation/*methods Pheromones/*analysis/chemistry Thuja/*chemistry Gc-ms Gas liquid microextraction Plant semiochemicals Quantification Thuja koraiensis Nakai;
Notes:		"MedlineZhao, Jinhua Jin, Xiangzi Wang, Xiaoping Yang, Cui Piao, Xiangfan Kaw, Han Yeong Li, Donghao eng Ireland 2020/08/11 Plant Sci. 2020 Sep; 298:110576. doi: 10.1016/j.plantsci.2020.110576. Epub 2020 Jun 23"