« Previous AbstractInvestigation of defect-rich CeO(2) catalysts for super low-temperature catalytic oxidation and durable styrene removal    Next AbstractMultidimensional profiling indicates the shifts and functionality of wheat-origin microbiota during high-temperature Daqu incubation »

J Agric Food Chem

Title:		MAPK Cascades Mediating Biocontrol Activity of Trichoderma brevicrassum Strain TC967
Author(s):		Zhang Y; Zhuang WY;
Address:		"State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. University of Chinese Academy of Sciences, Beijing 100049, China"
Journal Title:		J Agric Food Chem
Year:		2022
Volume:		20220222
Issue:		8
Page Number:		2762 - 2775
DOI:		10.1021/acs.jafc.1c05622
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Trichoderma brevicrassum strain TC967 is a novel biological control agent (BCA) against the plant pathogen Rhizoctonia solani and promotes plant growth. Mitogen-activated protein kinase (MAPK) cascades are involved in a variety of physiological functions of Trichoderma, but functions of each MAPK in regulating biocontrol have not been characterized in a single Trichoderma strain. In this study, we assembled and annotated the genome of strain TC967 and identified its three MAPK gene sequences. Functions of Fus3-, Slt2-, and Hog1-MAPK in strain TC967 were dissected. All three MAPKs were involved in hyphal growth. Hog1-MAPK was essential for conidiation and tolerance to saline/osmotic stress. Both Fus3- and Slt2-MAPK deletion mutants reduced cell-wall integrity (CWI) and increased the activities of chitinase and protease. The growth of Rhizoctoniasolani was further inhibited by volatile organic compounds (VOCs) and secondary metabolites produced by Fus3- and Slt2-MAPK deletion mutants, respectively. Biocontrol assays demonstrated that Fus3- and Slt2-MAPK deletion mutants were considerably more effective in disease control than the wild-type strain. RNA-seq analysis revealed that MAPK collectively played a major role in regulating biocontrol-related gene expressions, including the genes in charge of secondary metabolism, fungal cell wall-degrading enzymes (FCWDEs), and small secreted cysteine-rich proteins (SSCPs)"
Keywords:		*Hypocreales/metabolism MAP Kinase Signaling System Mitogen-Activated Protein Kinases/metabolism *Trichoderma Mapk Rhizoctonia solani Trichoderma biocontrol cucumber;
Notes:		"MedlineZhang, Yi Zhuang, Wen-Ying eng 2022/02/23 J Agric Food Chem. 2022 Mar 2; 70(8):2762-2775. doi: 10.1021/acs.jafc.1c05622. Epub 2022 Feb 22"