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Biomed Res Int

Title:		Anticancer Properties of Different Solvent Extracts of Cucumis melo L. Seeds and Whole Fruit and Their Metabolite Profiling Using HPLC and GC-MS
Author(s):		Zhang X; Bai Y; Wang Y; Wang C; Fu J; Gao L; Liu Y; Feng J; Swamy MK; Yogi M; Rudramurthy GR; Purushotham B; Deng Y;
Address:		"Encephalopathy Center, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin Province 130021, China. Extrathoracic and Thyroid Mammary Surgery, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin Province 130021, China. Department of Respiratory, First Clinical College of Chinese Academy of Traditional Chinese Medicine, Changchun City, Jilin Province 130021, China. Reproductive Center, Jilin Provincial People's Hospital, Changchun, Jilin Province 130021, China. Brain Surgery, Liaoyuan Hospital of Traditional Chinese Medicine, Liaoyuan, Jilin Province 136000, China. Department of Orthopedics, People's Hospital, Sanya, Hainan Province 572000, China. Department of Biotechnology, East West First Grade College of Science, Bengaluru 560091, India. Heart Disease Center, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin Province 130021, China"
Journal Title:		Biomed Res Int
Year:		2020
Volume:		20200224
Issue:
Page Number:		5282949 -
DOI:		10.1155/2020/5282949
ISSN/ISBN:		2314-6141 (Electronic) 2314-6133 (Print)
Abstract:		"Honeydew melon (Cucumis melo L.) is an oval-shaped delicious fruit of one cultivar group of the muskmelon with immense nutritional importance and is extensively consumed by many tropical countries. The effect of various organic solvents on the recovery of phytochemicals from honeydew melon plant fruits and seeds was assessed. Further, High-Performance Liquid Chromatography (HPLC) was used to examine and assess the contents of phenolic acid (gallic acid) and flavonoid (rutin) compounds. The use of gas chromatography-mass spectrometry (GC-MS) analysis explained the presence of volatile phytocompounds in the extracts. The use of organic solvents had a substantial impact on the total dry weight and extract yield. In general, the solvent-extracted constituents remained in the order of methanol>chloroform>distilled water for both honeydew melon seeds and whole fruit. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was used to assess the cytotoxicity effect against PC3, HCT116, HeLa, and Jurkat cell lines. The chloroform extract exhibited a good cytotoxic activity against all cell lines as compared to other solvent extracts. HPLC analysis revealed the occurrence of gallic acid content of 0.102 +/- 0.23 mg/10 mg of dry whole fruit extract, while 10 mg of dry seed extract contained only 0.022 +/- 0.12 mg of gallic acid content. Likewise, rutin content was observed to be 0.224 +/- 0.31 mg and 0.1916 +/- 0.82 mg/10 mg of dry whole fruit and seed extract, respectively. Further, GC-MS analysis revealed the presence of a total of 37 compounds in chloroform extract of whole fruit, while only 14 compounds were found in seed extract. Nevertheless, more examinations are needed to identify and characterize other metabolites from honeydew melon and evaluate their pharmacological importance"
Keywords:		"*Antineoplastic Agents, Phytogenic/chemistry/isolation & purification/pharmacology Cucumis melo/*chemistry HCT116 Cells HeLa Cells Humans Jurkat Cells Neoplasms/*drug therapy/metabolism/pathology PC-3 Cells *Plant Extracts/chemistry/isolation & purificati;"
Notes:		"MedlineZhang, Xudong Bai, Yuzhuo Wang, Yun Wang, Chunlan Fu, Jianhua Gao, Longlan Liu, Yu Feng, Jingbin Swamy, Mallappa Kumara Yogi, Maddipatla Rudramurthy, Gudepalya Renukaiah Purushotham, Boregowda Deng, Yue eng 2020/03/19 Biomed Res Int. 2020 Feb 24; 2020:5282949. doi: 10.1155/2020/5282949. eCollection 2020"