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J Insect Physiol

Title:		"The diapause hormone-pheromone biosynthesis activating neuropeptide gene of Helicoverpa armigera encodes multiple peptides that break, rather than induce, diapause"
Author(s):		Zhang TY; Sun JS; Zhang QR; Xu J; Jiang RJ; Xu WH;
Address:		"Department of Molecular and Cell Biology, University of Science and Technology of China, Hefei 230027"
Journal Title:		J Insect Physiol
Year:		2004
Volume:		50
Issue:		6
Page Number:		547 - 554
DOI:		10.1016/j.jinsphys.2004.03.011
ISSN/ISBN:		0022-1910 (Print) 0022-1910 (Linking)
Abstract:		"FXPRLamide peptides encoded by the DH-PBAN (diapause hormone-pheromone biosynthesis activating neuropeptide) gene induce embryonic diapause in Bombyx mori, but terminate pupal diapause in Helicoverpa armigera (Har). Here, we explore the mechanisms of terminating pupal diapause by the FXPRLamide peptides. Using quantitative RT-PCR, we observed that expression of Har-DH-PBAN mRNA in the SG of nondiapause-type pupae was significantly higher than in diapause-type pupae. Immunocytochemical results indicated that the level of FXPRLamide peptides and axonal release are related to the diapause decision. Ecdysteroidogenesis in prothoracic glands (PGs) was stimulated by synthetic Har-DH in vivo and in vitro, and labeled Har-DH bound to the membrane of the PG, thus suggesting that DH breaks diapause by activating the PG to synthesize ecdysone. Furthermore, the response of DH in terminating diapause was temperature dependent. Decerebration experiments showed that the brain can control pupal development through the regulation of DH, and DH can terminate diapause and promote development without the brain. This result suggests a possible mechanism of response for the signals of DH and other FXPRLamide peptides in H. armigera"
Keywords:		"Animals Ecdysone/metabolism Ganglia, Invertebrate/metabolism Hibernation/genetics/*physiology Insect Hormones/genetics/*metabolism Metamorphosis, Biological/genetics/*physiology Moths/genetics/*growth & development/metabolism Neuropeptides/genetics/*metab;"
Notes:		"MedlineZhang, Tian-Yi Sun, Jiu-Song Zhang, Qi-Rui Xu, Jun Jiang, Rong-Jing Xu, Wei-Hua eng Research Support, Non-U.S. Gov't England 2004/06/09 J Insect Physiol. 2004 Jun; 50(6):547-54. doi: 10.1016/j.jinsphys.2004.03.011"