| Title: | Characterization of three pheromone-binding proteins (PBPs) of Helicoverpa armigera (Hubner) and their binding properties |
| Author(s): | Zhang TT; Mei XD; Feng JN; Berg BG; Zhang YJ; Guo YY; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China" |
| DOI: | 10.1016/j.jinsphys.2012.04.010 |
| ISSN/ISBN: | 1879-1611 (Electronic) 0022-1910 (Linking) |
| Abstract: | "Three pheromone-binding proteins of Helicoverpa armigera were cloned and expressed in Escherichia coli. In order to characterize their physiological properties, ligand-binding experiments were performed using five biologically relevant substances including sex pheromones and interspecific signals. The results showed that one of the pheromone-binding proteins, HarmPBP1, binds strongly to each of the two principal pheromone components of H. armigera, (Z)-11-tetradecenal and (Z)-9-hexadecenal, but not to the interspecific signal (Z)-9-tetracecenal. The two remaining pheromone-binding proteins, HarmPBP2 and HarmPBP3, showed only weak affinities with the ligands tested. The 3-D structure of HarmPBP1 was predicted and the docking experiments indicate that the key binding site of (Z)-9-hexadecenal to HarmPBP1 includes Thr112, Lys111, and Phe119 whereas that of (Z)-11-tetradecenal includes Ser9, Trp37, Phe36, and Phe119" |
| Keywords: | Amino Acid Sequence Animals Carrier Proteins/chemistry/genetics/*metabolism Insect Proteins/chemistry/genetics/*metabolism Kinetics Molecular Sequence Data Moths/chemistry/genetics/*metabolism Pheromones/*metabolism Protein Binding Sequence Alignment; |
| Notes: | "MedlineZhang, Tian-Tao Mei, Xiang-Dong Feng, Ji-Nian Berg, Bente G Zhang, Yong-Jun Guo, Yu-Yuan eng Research Support, Non-U.S. Gov't England 2012/05/03 J Insect Physiol. 2012 Jul; 58(7):941-8. doi: 10.1016/j.jinsphys.2012.04.010. Epub 2012 Apr 28" |
