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Methods Mol Biol

Title:		Headspace SPME-GC-MS metabolomics analysis of urinary volatile organic compounds (VOCs)
Author(s):		Zhang S; Raftery D;
Address:		"Department of Neurosurgery, Nutrition Research Institute, Chapel Hill School of Public Health, Brigham and Women's Hospital, Boston, Massachusetts, USA, szhang17@partners.org"
Journal Title:		Methods Mol Biol
Year:		2014
Volume:		1198
Issue:
Page Number:		265 - 272
DOI:		10.1007/978-1-4939-1258-2_17
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"In this chapter, we describe the procedure for metabolic profiling of hundreds of urinary volatile organic compounds (VOCs) using headspace solid-phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS). SPME is a solvent-free technology that is reproducible, fast, cost effective, and versatile in extracting small molecular weight organic compounds (metabolites) from biofluids using a fiber that is coated with an extracting phase. After extraction, the SPME fiber can be directly injected to the GC-MS, where the extracted metabolites will desorb thermally from the fiber, elute along a GC column, and finally enter into the MS for detection. The analysis of urine samples is presented using this approach"
Keywords:		Gas Chromatography-Mass Spectrometry/*methods Humans Metabolomics/*methods Solid Phase Microextraction/*methods Urinalysis/methods Volatile Organic Compounds/isolation & purification/*metabolism/*urine;
Notes:		"MedlineZhang, Shucha Raftery, Daniel eng Research Support, Non-U.S. Gov't 2014/10/02 Methods Mol Biol. 2014; 1198:265-72. doi: 10.1007/978-1-4939-1258-2_17"